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Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Based on the graph below, write a discussion in detail

Procedure Take ml Top up to 50ml. Add 50 μι of Mix Sml mixture to Add fresh LB ampicillin to the overnight culture with 45 ml LB mixture analyse OD600 the both broth Measure every 15 mins. Stop until 75 mins. The mixture is then incubated Withdraw 3ml mixture to analyse (OD600)record as zero time-point. Overnight culture (E-coli + LB borth) at shaked incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Take 3 ml No ampicillin No ampicilin mixture analyse OD600 to the broth The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until incubated incubator at 37°c 200rpm shaked analvse (OD600)record as zero time-point. at 75 mins. Graph of Absorbance vs Time(mins) 14 12 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1(Ampicilin) Group 1(Control) Measurement of bacterial cell growth in LB Absorbance(600nm)
Transcribed Image Text:Procedure Take ml Top up to 50ml. Add 50 μι of Mix Sml mixture to Add fresh LB ampicillin to the overnight culture with 45 ml LB mixture analyse OD600 the both broth Measure every 15 mins. Stop until 75 mins. The mixture is then incubated Withdraw 3ml mixture to analyse (OD600)record as zero time-point. Overnight culture (E-coli + LB borth) at shaked incubator at 37°c 200rpm Mix overnight culture B (control) with 45 ml LB Sml Take 3 ml No ampicillin No ampicilin mixture analyse OD600 to the broth The mixture is then Withdraw 3ml mixture to Measure every 15 mins. Stop until incubated incubator at 37°c 200rpm shaked analvse (OD600)record as zero time-point. at 75 mins. Graph of Absorbance vs Time(mins) 14 12 0.8 0.6 0.4 0.2 10 20 30 40 50 60 70 80 Time (mins) Group 1(Ampicilin) Group 1(Control) Measurement of bacterial cell growth in LB Absorbance(600nm)
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