Make a flowchart: POUR PLATE METHOD

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Make a flowchart: POUR PLATE METHOD

g. Using a pipet, transfer 1 ml from each tube to a duplicate sterile plates. Pour melted
afar and mix as in the below.
NOTEOR
A diagram for mixing inoculum and melted medium.
h. Allow the agar to solidify and incubate as in Activity 4.
i. Examine after 48 hours.
Transcribed Image Text:g. Using a pipet, transfer 1 ml from each tube to a duplicate sterile plates. Pour melted afar and mix as in the below. NOTEOR A diagram for mixing inoculum and melted medium. h. Allow the agar to solidify and incubate as in Activity 4. i. Examine after 48 hours.
B: POUR PLATE METHOD:
a. Liquefy medium in a water bath.
b. Obtain three 9-ml water blanks in screw cap tubes and label each tube from 1 to 3.
c. Secure a broth mix culture from your instructor.
d. With a sterile pipet, transfer one ml from the stock culture to tube #1. Cover and shake
vigorously.
e. From tube #1, transfer 1 ml to tube #2. Mix.
f. Again transfer 1 ml from tube #2 to tube #3. Shake and mix
1 ml
1ml
1 ml
mix culture
1:10
1:100
1:1000
A scheme for preparation of serial dilution.
g. Using a pipet, transfer 1 ml from each tube to a duplicate sterile plates. Pour melted
afar and mix as in the below.
Transcribed Image Text:B: POUR PLATE METHOD: a. Liquefy medium in a water bath. b. Obtain three 9-ml water blanks in screw cap tubes and label each tube from 1 to 3. c. Secure a broth mix culture from your instructor. d. With a sterile pipet, transfer one ml from the stock culture to tube #1. Cover and shake vigorously. e. From tube #1, transfer 1 ml to tube #2. Mix. f. Again transfer 1 ml from tube #2 to tube #3. Shake and mix 1 ml 1ml 1 ml mix culture 1:10 1:100 1:1000 A scheme for preparation of serial dilution. g. Using a pipet, transfer 1 ml from each tube to a duplicate sterile plates. Pour melted afar and mix as in the below.
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