Robert Bost and Richard Cribbs studied a strain of E. coli (araB14)that possessed a nonsense mutation in the structural gene that encodes Lribulokinase,an enzyme that allows the bacteria to metabolize the sugararabinose (R. Bost and R. Cribbs. 1969. Genetics 62:1–8). From thearaB14 strain, they isolated some bacteria that possessed mutations thatcaused them to revert back to the wild type. Genetic analysis of theserevertants showed that they possessed two different suppressormutations. One suppressor mutation (R1) was linked to the originalmutation in L-ribulokinase and probably occurred at the same locus. Byitself, this mutation allowed the production of L-ribulokinase, but theenzyme produced was not as effective in metabolizing arabinose as theenzyme encoded by the wild-type allele. The second suppressormutation (SuB) was not linked to the original mutation. In conjunctionwith the R1 mutation, SuB allowed the production of L-ribulokinase, butSuB by itself was not able to suppress the original mutation. intragenic suppressors or intergenic suppressors? Explain your reasoning. b. Propose an explanation for how R1 and SuB restore the ability of araB14 to metabolize arabinose and why SuB is able to more fully restore this ability.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
Robert Bost and Richard Cribbs studied a strain of E. coli (araB14)
that possessed a nonsense mutation in the structural gene that encodes Lribulokinase,
an enzyme that allows the bacteria to
arabinose (R. Bost and R. Cribbs. 1969. Genetics 62:1–8). From the
araB14 strain, they isolated some bacteria that possessed mutations that
caused them to revert back to the wild type. Genetic analysis of these
revertants showed that they possessed two different suppressor
mutations. One suppressor mutation (R1) was linked to the original
mutation in L-ribulokinase and probably occurred at the same locus. By
itself, this mutation allowed the production of L-ribulokinase, but the
enzyme produced was not as effective in metabolizing arabinose as the
enzyme encoded by the wild-type allele. The second suppressor
mutation (SuB) was not linked to the original mutation. In conjunction
with the R1 mutation, SuB allowed the production of L-ribulokinase, but
SuB by itself was not able to suppress the original mutation. intragenic suppressors or intergenic suppressors? Explain your reasoning.
b. Propose an explanation for how R1 and SuB restore the ability of
araB14 to metabolize arabinose and why SuB is able to more fully restore
this ability.
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