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Read the scenario presented in the picture. The conditions employed in laboratory settings could mimic the outcomes of prolonged usage of tamoxifen and hormone deprivation therapy. We don't know much about the phenotypic profile of these newly emerged cancer cells. How might you explain the lack of response of TamC3 to tamoxifen treatment and TamR3 to estrogen deprivation?

Read the scenario presented in the picture. The conditions employed in laboratory settings could mimic the outcomes of prolonged usage of tamoxifen and hormone deprivation therapy. We don't know much about the phenotypic profile of these newly emerged cancer cells. How might you explain the lack of response of TamC3 to tamoxifen treatment and TamR3 to estrogen deprivation?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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Read the scenario presented in the picture. The conditions employed in laboratory settings could mimic the outcomes of prolonged usage of tamoxifen and hormone deprivation therapy. We don't know much about the phenotypic profile of these newly emerged cancer cells. How might you explain the lack of response of TamC3 to tamoxifen treatment and TamR3 to estrogen deprivation?
Part III - Generating TamC3 and TamR3 Cell Lines As Emily, Eric, and Lena entered the conference room, they found Dr. Mendoza helping himself to coffee and a doughnut, seated at a conference table with a couple of graduate students. "Dr. Mendoza, I would like you to meet Emily, our internship student from Alfred, New York. Dr. Mendoza is an oncologist, and he works at the clinic. We collaborate," said Eric. "Hi Emily! Nice to meet you. Would you like a cup of coffee?" "Hello Dr. Mendoza, yes. Thank you." "You've already met the rest of the group. I gather they took you out last night. Did you guys have fun?" Eric smiled as he looked at Mike and Jen, the graduate students in his lab. "Yes boss! We need more time off like this," replied Mike. "OK, who is presenting first today?" asked Eric. "I'm up," said Mike coming up to the white screen, where the PowerPoint presentation with the experimental results would be displayed. Lena dimmed the lights and Mike started his presentation. "Some breast cancer cells can develop resistance to the tamoxifen treatment and can survive the estrogen deprivation conditions. The mechanisms of the drug and hormone deprivation resistance are not known. To investigate this phenomenon, we took the MCF-7 cells into the laboratory" "Did I you try to make the cell resistant to tamoxifen?" asked Dr. Mendoza. "Yes. We divided them into two groups. One batch of the cells was exposed to tamoxifen for some period of time, which we did to mimic the clinical condition for the tamoxifen treatment in patients. Some of the cells died during the course of the experiment, but those that survived were selected and cultivated further." "Wow!" interrupted Eric. "So in this way you generated a new cell line. What did you call it?" "We're calling it TamC3. These cells were resistant to tamoxifen. The other batch of the MCF-7 cells was subjected to conditions without estrogen." "Very clever!" this time Dr. Mendoza interrupted. "That's mimick- ing the condition in patients subjected to hormone deprivation therapy. And what happened?" "The cells that survived the estrogen deprivation established another cell line, TamR3. Those cells are estrogen independent. Look at this graph," replied Mike (Figure 1). TamC3 Tamoxifen MCF-7 Estrogen deprivation TamR3 Figure 1. Generation of tamoxifen resistant (TamC3) and estrogen-independent (TamR3) cell lines.
Transcribed Image Text:Part III - Generating TamC3 and TamR3 Cell Lines As Emily, Eric, and Lena entered the conference room, they found Dr. Mendoza helping himself to coffee and a doughnut, seated at a conference table with a couple of graduate students. "Dr. Mendoza, I would like you to meet Emily, our internship student from Alfred, New York. Dr. Mendoza is an oncologist, and he works at the clinic. We collaborate," said Eric. "Hi Emily! Nice to meet you. Would you like a cup of coffee?" "Hello Dr. Mendoza, yes. Thank you." "You've already met the rest of the group. I gather they took you out last night. Did you guys have fun?" Eric smiled as he looked at Mike and Jen, the graduate students in his lab. "Yes boss! We need more time off like this," replied Mike. "OK, who is presenting first today?" asked Eric. "I'm up," said Mike coming up to the white screen, where the PowerPoint presentation with the experimental results would be displayed. Lena dimmed the lights and Mike started his presentation. "Some breast cancer cells can develop resistance to the tamoxifen treatment and can survive the estrogen deprivation conditions. The mechanisms of the drug and hormone deprivation resistance are not known. To investigate this phenomenon, we took the MCF-7 cells into the laboratory" "Did I you try to make the cell resistant to tamoxifen?" asked Dr. Mendoza. "Yes. We divided them into two groups. One batch of the cells was exposed to tamoxifen for some period of time, which we did to mimic the clinical condition for the tamoxifen treatment in patients. Some of the cells died during the course of the experiment, but those that survived were selected and cultivated further." "Wow!" interrupted Eric. "So in this way you generated a new cell line. What did you call it?" "We're calling it TamC3. These cells were resistant to tamoxifen. The other batch of the MCF-7 cells was subjected to conditions without estrogen." "Very clever!" this time Dr. Mendoza interrupted. "That's mimick- ing the condition in patients subjected to hormone deprivation therapy. And what happened?" "The cells that survived the estrogen deprivation established another cell line, TamR3. Those cells are estrogen independent. Look at this graph," replied Mike (Figure 1). TamC3 Tamoxifen MCF-7 Estrogen deprivation TamR3 Figure 1. Generation of tamoxifen resistant (TamC3) and estrogen-independent (TamR3) cell lines.
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