Homework Help is Here – Start Your Trial Now!
Science
Biology
Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end).

Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end).

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 .. 9197) pRS-marker Nrul (140) (9132) SgrDI (9015) Sspl Mlul (160) Spel (181) Ndel (416) SnaBI (522) (8761 .. 8780) Amp-R (8581) Pvul CMV-F (701.. 721) Eco53kl (748) Sacl (750) 9000 T7 (795 .. 814) Nhel (827) Bmtl (831) BsmBI (873) CMV en... 1000 CMV promoter T7 promoter (7459 . 7478) pBR322ori-F (7318) Pcil (7208 .. 7225) L4440 (6990 . 7012) M13/pUC Reverse (6977 . 6993) M13 Reverse pcDNA3.1- 9202 bp (6939) BstZ17I (6898 . 6917) EBV-rev (6887) Bsml (6844 .. 6863) SV40PA-R (6649) BSTBI 2000 M13 rev SV40 poly 10008 1000L-
Transcribed Image Text:Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 .. 9197) pRS-marker Nrul (140) (9132) SgrDI (9015) Sspl Mlul (160) Spel (181) Ndel (416) SnaBI (522) (8761 .. 8780) Amp-R (8581) Pvul CMV-F (701.. 721) Eco53kl (748) Sacl (750) 9000 T7 (795 .. 814) Nhel (827) Bmtl (831) BsmBI (873) CMV en... 1000 CMV promoter T7 promoter (7459 . 7478) pBR322ori-F (7318) Pcil (7208 .. 7225) L4440 (6990 . 7012) M13/pUC Reverse (6977 . 6993) M13 Reverse pcDNA3.1- 9202 bp (6939) BstZ17I (6898 . 6917) EBV-rev (6887) Bsml (6844 .. 6863) SV40PA-R (6649) BSTBI 2000 M13 rev SV40 poly 10008 1000L-
pcDNA3.1- 9202 bp (6939) BstZ17I (6898 . 6917) EBV-rev (6887) Bsml (6844 .. 6863) SV40PA-R (6649) BstBI (6503 . 6522) Neo-F (6483) Rsrll SV40 ori (6364) BSSHII K PpuMI (3289) (5893 . 5912) Neo-R bGH poly(A) signal (5780) Smal (5778) TspMI - Xmal (5757) Avrll (5756) Stul | 4000 5000 (5685 . 5704) SV40pro-F BbvCI (3798) (5438 . 5458) PBABE 3' (5298 . 5319) Flori-F (5088 . 5107) Flori-R (4725 . 4742) BGH-rev (4709) Pmel (4704) Apal (4700) PspOMI PaeR7I - PspXI - Xhol (4688) Xbal (4694) 000E M13 re e sv40 poly(A) signal NeoR/KanR 000L 16000 O promoter o
Transcribed Image Text:pcDNA3.1- 9202 bp (6939) BstZ17I (6898 . 6917) EBV-rev (6887) Bsml (6844 .. 6863) SV40PA-R (6649) BstBI (6503 . 6522) Neo-F (6483) Rsrll SV40 ori (6364) BSSHII K PpuMI (3289) (5893 . 5912) Neo-R bGH poly(A) signal (5780) Smal (5778) TspMI - Xmal (5757) Avrll (5756) Stul | 4000 5000 (5685 . 5704) SV40pro-F BbvCI (3798) (5438 . 5458) PBABE 3' (5298 . 5319) Flori-F (5088 . 5107) Flori-R (4725 . 4742) BGH-rev (4709) Pmel (4704) Apal (4700) PspOMI PaeR7I - PspXI - Xhol (4688) Xbal (4694) 000E M13 re e sv40 poly(A) signal NeoR/KanR 000L 16000 O promoter o
Expert Solution
steps

Step by step

Solved in 5 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Genome annotation
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS