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Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end).

Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end).

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 .. 9197) pRS-marker Nrul (140) (9132) SgrDI (9015) Sspl Mlul (160) Spel (181) Ndel (416) SnaBI (522) (8761 .. 8780) Amp-R (8581) Pvul CMV-F (701.. 721) Eco53kl (748) Sacl (750) 9000 T7 (795 .. 814) Nhel (827) Bmtl (831) BsmBI (873) CMV en... 1000 CMV promoter T7 promoter (7459 . 7478) pBR322ori-F (7318) Pcil (7208 .. 7225) L4440 (6990 . 7012) M13/pUC Reverse (6977 . 6993) M13 Reverse pcDNA3.1- 9202 bp (6939) BstZ17I (6898 . 6917) EBV-rev (6887) Bsml (6844 .. 6863) SV40PA-R (6649) BSTBI 2000 M13 rev SV40 poly 10008 1000L-
Transcribed Image Text:Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the end of the gene (N- terminus end). (9178 .. 9197) pRS-marker Nrul (140) (9132) SgrDI (9015) Sspl Mlul (160) Spel (181) Ndel (416) SnaBI (522) (8761 .. 8780) Amp-R (8581) Pvul CMV-F (701.. 721) Eco53kl (748) Sacl (750) 9000 T7 (795 .. 814) Nhel (827) Bmtl (831) BsmBI (873) CMV en... 1000 CMV promoter T7 promoter (7459 . 7478) pBR322ori-F (7318) Pcil (7208 .. 7225) L4440 (6990 . 7012) M13/pUC Reverse (6977 . 6993) M13 Reverse pcDNA3.1- 9202 bp (6939) BstZ17I (6898 . 6917) EBV-rev (6887) Bsml (6844 .. 6863) SV40PA-R (6649) BSTBI 2000 M13 rev SV40 poly 10008 1000L-
pcDNA3.1- 9202 bp (6939) BstZ17I (6898 . 6917) EBV-rev (6887) Bsml (6844 .. 6863) SV40PA-R (6649) BstBI (6503 . 6522) Neo-F (6483) Rsrll SV40 ori (6364) BSSHII K PpuMI (3289) (5893 . 5912) Neo-R bGH poly(A) signal (5780) Smal (5778) TspMI - Xmal (5757) Avrll (5756) Stul | 4000 5000 (5685 . 5704) SV40pro-F BbvCI (3798) (5438 . 5458) PBABE 3' (5298 . 5319) Flori-F (5088 . 5107) Flori-R (4725 . 4742) BGH-rev (4709) Pmel (4704) Apal (4700) PspOMI PaeR7I - PspXI - Xhol (4688) Xbal (4694) 000E M13 re e sv40 poly(A) signal NeoR/KanR 000L 16000 O promoter o
Transcribed Image Text:pcDNA3.1- 9202 bp (6939) BstZ17I (6898 . 6917) EBV-rev (6887) Bsml (6844 .. 6863) SV40PA-R (6649) BstBI (6503 . 6522) Neo-F (6483) Rsrll SV40 ori (6364) BSSHII K PpuMI (3289) (5893 . 5912) Neo-R bGH poly(A) signal (5780) Smal (5778) TspMI - Xmal (5757) Avrll (5756) Stul | 4000 5000 (5685 . 5704) SV40pro-F BbvCI (3798) (5438 . 5458) PBABE 3' (5298 . 5319) Flori-F (5088 . 5107) Flori-R (4725 . 4742) BGH-rev (4709) Pmel (4704) Apal (4700) PspOMI PaeR7I - PspXI - Xhol (4688) Xbal (4694) 000E M13 re e sv40 poly(A) signal NeoR/KanR 000L 16000 O promoter o
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