If you didn't know the sequence of the segment of DNA you want to amplify, how would you perform the PCR reaction? You would need to first isolate the mRNA made from the segment of DNA, then use the MRNA as a primer for the PCR You would have to increase the amount of DNA you wish to amplify or the reaction will not proceed You would synthesize primers in the lab to use for the PCR reaction You would not be able to perform the PCR reaction on this segment of DNA After heat denaturing the segment of DNA, you can add more of the DNA segment to serve as primers
If you didn't know the sequence of the segment of DNA you want to amplify, how would you perform the PCR reaction? You would need to first isolate the mRNA made from the segment of DNA, then use the MRNA as a primer for the PCR You would have to increase the amount of DNA you wish to amplify or the reaction will not proceed You would synthesize primers in the lab to use for the PCR reaction You would not be able to perform the PCR reaction on this segment of DNA After heat denaturing the segment of DNA, you can add more of the DNA segment to serve as primers
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
Do question 35
Transcribed Image Text:If you didn't know the sequence of the segment of DNA you want to amplify, how
would you perform the PCR reaction?
You would need to first isolate the mRNA made from the segment of DNA, then use the
MRNA as a primer for the PCR
You would have to increase the amount of DNA you wish to amplify or the reaction will not
proceed
You would synthesize primers in the lab to use for the PCR reaction
You would not be able to perform the PCR reaction on this segment of DNA
After heat denaturing the segment of DNA, you can add more of the DNA segment to serve
as primers
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 4 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education