Question 21 Your research team has been tasked with using a variety of horizontal gene transfer techniques to determine the overall order of genes on the bacterial chromosome in the R. ibiscus prokaryote usin, growth of bacteria. Growth in media is the byproduct of cell division and thus DNA replication precedes any kind of growth that we might observe. Select the statement below that is true of prokaryotic DNA replication. O it requires only DNA polymerase; no primase is necessary O it begins at multiple origins of replication throughout the bacterial chromosome O it begins at one origin of replication on the bacterial chromosome telomerase is needed to break the hydrogen bonds between DNA strands Question 22 Now that we've got that settled; let's start looking at some data. You decided to begin with some interrupted mating experiments to determine chromosomal location of various genes that encode biological factors necessary for survival. You began with a donor Hfr R. ibiscus that had a genotype of acai* blue* pear* rasp*. Your recipient F- R. ibiscus has a genotype of acai blue pear rasp". What type of horizontal gene transfer are you hoping for with this experimental set up? O transduction O transformation
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
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