Question 8 Electrophoresis in agarose gel A The DNA will migrate to the negative elec- trode due to the presence of phosphate groups. B For a given DNA molecule, the higher the percentage in G/C pairs, the less the DNA will migrate. C The migration speed depends on the agarose percentage. D The migration of an undigested, circular plasmid DNA will depend solely on its number of base pairs. E If the migration buffer is at physiological pH (7.4), the DNA will not migrate.

Question 8 Electrophoresis in agarose gel A The DNA will migrate to the negative elec- trode due to the presence of phosphate groups. B For a given DNA molecule, the higher the percentage in G/C pairs, the less the DNA will migrate. C The migration speed depends on the agarose percentage. D The migration of an undigested, circular plasmid DNA will depend solely on its number of base pairs. E If the migration buffer is at physiological pH (7.4), the DNA will not migrate.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 5 Imagine that you have cloned the gene encoding the SARS-CoV-2 spike protein to a plasmid vector. The next thing is to put the plasmid into a host. Describe how you can transform the plasmid containing the gene encoding the SARS-CoV-2 spike protein.
question 6
Question 10 Produce the complimentary DNA strand that would be matched with the provided strand T--A C-- G C-- G A -- A -- C-- G-- T-- T-- A -- C-- G -- G--
QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…
QUESTION 2 A linear piece of DNA is cut as follows: cut with Ecorl - get 1kb and 4 kb band Cut with Bamhl - get 2 kb and3 kb bands Cut with both Bamhl and Ecorl - get 1kb, 2 kb and 2 kb band From this we can conclude that the distance between the Bamhl site and the Ecorl site is O A1 kb O B. 2 kb O C. 3kb O D. 4kb O E. 5 kb
QUESTION 5 If DNA had 5 different bąses, a restriction enzyme with a 3-base recognition sequence would cut DNA approximately every O A 125 bp. O B. 256 bp. O C. 425 bp. O D. 625 bp. O E. 3125 bp.
QUESTION 9 Imagine that a researcher has a cell from a genetically engineered organism and a clone of that same organism. The researcher uses PCR and then rruns a DNA fingerprint on the two samples. What should the researcher find on the gel? O A. The gel will be blank because the heat from the PCR will destroy the DNA. O B. The gel will be blank because the heat from the PCR will change the charge of the DNA and cause it to run in the opposite direction, off of the gel and into the buffer. OC. The DNA banding pattern for the two organisms will be identical. O D. The DNA banding pattern for the two organisms will be different because every organism has original DNA. QUEST ION 10
QUESTION 3 Is it possible to dissolve DNA in water? a. Only during cell division b. No, DNA is soluble only in lipids c. Yes, DNA is polar due to the presence of sugars and charged phosphate groups d. In ancient times yes, however, DNA became non-water soluble very recently. QUESTION 4 What are the solutions to extract DNA in your experiment? a. hydrochloric acid; aniline b. water, oil c. Biuret; water d. water; ethanol
Question 5 Review DNA sequencing and cloning tools. Which of these is not used to make a recombinant DNA? O restriction enzymes to create sticky ends of a plasmid O fragment from a different DNA cut by the same restriction enzyme O DNA ligase seals the recombinant DNA O denaturation
Question 4. You are analyzing cells that you have grown on coverslips and fixed. What method would you use to determine where in the cell a protein is located? A. Western blot B. SDS-PAGE C. Microarray D. Immnocytochemstry E. Single cell RNA sequencing
QUESTION 5 In the Sanger method of DNA sequencing, if a dideoxynucleotide is attached, a synthesising chain will? O a. Grow quicker b. Stop growing O c. Grow slower O d. Hybridise
QUESTION 20 Match the lettered activities to the following enzymes. Remember that some enzymes have multiple activities. A. 5' to 3' polymerase activity B. 3' to 5' DNA polymerase activity C. RNA-dependent DNA polymerase activity D. DNA dependent DNA polymerase activity E. 3' to 5' exonuclease activity F. 5' to 3' exonuclease activity DNA polymerase II DNA polymerase I telomerase