QUESTION 15 The researchers Hershey and Chase conducted an experiment where they radioactively labeled the proteins of a T2 phage with 35S. In a separate treatment, they labeled the DNA of a T2 phage with 32p. They allowed the labeled T2 phage to infect E. coli and then agitated the cells so that the phage would fall off of the cells. The cells were then spun down to the bottom of the tubes. When the proteins were labeled, did the researchers see radioactivity in the pelleted cells or the supernatant (liquid on top of pellet)? When the DNA was labeled, did the researchers see radioactivity in the pelleted cells or the supernatant? What conclusion did Hershey and Chase draw from this experiment?

QUESTION 15 The researchers Hershey and Chase conducted an experiment where they radioactively labeled the proteins of a T2 phage with 35S. In a separate treatment, they labeled the DNA of a T2 phage with 32p. They allowed the labeled T2 phage to infect E. coli and then agitated the cells so that the phage would fall off of the cells. The cells were then spun down to the bottom of the tubes. When the proteins were labeled, did the researchers see radioactivity in the pelleted cells or the supernatant (liquid on top of pellet)? When the DNA was labeled, did the researchers see radioactivity in the pelleted cells or the supernatant? What conclusion did Hershey and Chase draw from this experiment?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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QUESTION 14 The Hershey and Chase experiments, in which radioactive phosphorus (32P) and radioactive sulfur (35S) were used, demonstrated that O a. DNA labeled with 32P is transferred from the bacteriophage to the virus O b. DNA may be the hereditary material, although bacteriophages transfer both DNA and proteins into their hosts O C. proteins labeled with 35S become deactivated and unable to be transferred O d. DNA labeled with 35S and proteins labeled with 32P can be traced over the course of an experiment O e. bacteriophages transfer their DNA, not their coat proteins, into their hosts QUESTION 15 Which of the following makes its own food using energy and simple raw materials it obtains from nonbiological sources? O a. Cell O b. Producer O C. Protist O d. Consumer O e. Nutrient QUESTION 16 A salt will dissolve in water to form O a. bases O b. acids O c. buffers O d. ions other than H* and OH" O e. only hydrogen and oxygen bonds
QUESTION 3 You are provided with plasmid DNA at 200ng/uL, 10X restriction digestion buffer, restriction enzymes (1 unit/ul) and water. You want to digest 1.5 ug of plasmid DNA with 1 unit (U) of enzyme in 1X buffer in a total volume of 80 ul. Complete the table below: Solution Stock solution Working solution Volume needed restriction digestion buffer 10X 1X DNA 200 ng/ul 1.5 με enzymes 1 unit/ul 1 unit Water Total 80 μL
QUESTION 27 Which of the following methods can be used to compare the amounts of one specific mRNA that is expressed by two different cell lines? A. Immunohistochemistry B. Western blotting C. Polymerase chain reaction (PCR) D. Immunocytochemistry
Question #3: CRISPR has been used to cure an individual from sickle cell. Below is a Sanger electropherogram of a sequence from a patient without sickle cell and one with sickle cell. Sequence from a normal individual mmmm Sequence from the diseased individual G T GIIC A GC A Se SCIENCEphe A G A SCIENCE SCIENCEphoto G a) Where is the change in the sequence and what is the consequence to the protein sequence of this mutation? b) Below is an image of the normal and diseased quaternary hemoglobin protein. What is different about the protein shape and why does that structure have a huge impact on its function (please name the function!)? Adult haemogBRAR G G G G A G Sickle Cell haemoglobin S Structure a s RARY COLIBRARY c) If you were to use CRISPR to modify the genome of a diseased individual, to which nucleotides might you design your guide RNA? Why? d) RNA Seq is used to determine off-target effects of Cas9 cleavage. Why is this an appropriate tool to determine these effects? e) Data on…
QUESTION 1 In this gel, PCR is performed using primers outside of the repeat area anf the product is then run on a gel. Which of these alleles would be the lowest band and which would be the highest band when run on a gel (remember how electrophoresis works!!) ALLELES #1 -CACACACACACACACACACACACACACACA -CACACACACACACACACACACACACACACACACA # 2 # 3 -CACACACACACACACACACACACACACACACACACA- GENOTYPES 1 2 3 4 5 6. O 1 is lowest band and 3 is the highest band O 3 is lowest band and 1 is the highest band O 1 is the lowest band and 2 is the highest band
Question 16 The process of transformation in bacteria would best be described as bacterial cells are infected by a phage DNA molecule. external DNA is taken into a cell, becoming part of the cell's genome. a strand of DNA is created from an RNA molecule. a strand of RNA is created from a DNA molecule. Moving to another question will save this response.
QUESTION 13 A scientist would like to overexpress a lysosomal acid hydrolase in a cultured cell line as a way to investigate a potential therapy for a lysosomal storage disease. How might this scientist introduce a plasmid encoding this lysosomal enzyme into cells? O Scanning electron microscopy O Western blot analysis O Transfection with a lipid reagent (AKA liposomes) O Immunohistochemistry O Generate an organoid QUESTION 14 Heterotrimeric G-proteins that associate with the G-protein coupled receptors (GPCRs) consist of which of the following? OA. beta subunits C B. O C. alpha subunits alpha, beta, and gamma subuni OD. beta and gamma subunits O E. gamma subunits QUESTION 15 The activation of a common effector molecule in the cytoplasm by signals from a variety of unrelated receptors is called OA. Divergence OB. Convergence OC. Crosstalk OD. Transvergence QUESTION 16
Question 10 Produce the complimentary DNA strand that would be matched with the provided strand T--A C-- G C-- G A -- A -- C-- G-- T-- T-- A -- C-- G -- G--
QUESTION 2 The SARS-COV-2 pandemic resulted in the desire to design ELISA assays to either detect antibodies against SARS-CoV-2 protein (previous infection), or detect viral protein (current infection). You plan to express the mutant spike protein variant using the plasmid pictured below as your template. Here's the sequence you want to put in its place: https://www.ncbi.nlm.nih.gov/nuccore/AY429073.1?report=fasta, this is the DNA sequence you're going to buy as a gene block! But you need to add a couple things to it first so it'll get into the plasmid. To do so, you're going to need to remove the current insert (represented by the yellow arrow beginning at position 833-4867). Question: What restriction sites are you going to add at the ends of the gene? Answer can be in nucleotides or the enzyme name. For the first blank fill in the site name/sequence you'd add at the beginning of the gene (5' / C-terminus end) and for the second blank fill in the sequence/site name you'd add at the…
QUESTION 22 Choose all the experiment(s) from the list below that demonstrated that RNA is involved in a transfer step between DNA and protein. O A. pulse chase experiments using radioactive deoxyribose O B. Brenner and Crick's characterization of frameshift suppressor mutations O C. analysis of the burst of RNA synthesis after bacteriophage infection of E. coli O D. pulse chase experiments using radioactive uracil bases O E. the Meselson Stahl experiment
Question 18 Match Column A with Column B. transports amino acids to the site of protein synthesis A. operator B. rRNA has anticodon loop C. IRNA v directs amino acid sequence of proteins D. MRNA contains the Shine-Delgarno sequence E. most type of RNA v synthesized in the eukaryote nucleus F. promoter v found inside the ribosome v region where the RNA binds to the DNA template region where the activator protein binds to the DNA template
Question 16 Why can't SNPS be detected by PCR and Gel Electrophoresis? O Because Gel Electrophoresis detects size differences in DNA and SNPS do not change the size of the DNA strand. O Because SNPS cause deletions so large that they are beyond the limits of this technique to detect. O Because SNPS affect proteins and PCR only works on DNA. O Because SNPS are too complicated to detect with this technology.