Which of the following methods can be used to compare the amounts of one specific mRNA that is expressed by two different cell lines? A. Immunohistochemistry B. Western blotting C. Polymerase chain reaction (PCR) D. Immunocytochemistry
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QUESTION 27
-
Which of the following methods can be used to compare the amounts of one specific mRNA that is expressed by two different cell lines?
A. Immunohistochemistry
B. Western blotting
C. Polymerase chain reaction (PCR)
D. Immunocytochemistry
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- Question 3. Biochemistry techniques have been used for the analysis on biomolecules such as enzymes, DNA, antibodies and other molecules in biomedical engineering. b) ELISA is a plate-based assay technique, designed for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. Examine two principles of ELISA. c) Gel electrophoresis is a laboratory technique, used to separate the mixture of macro- molecules such as DNA, RNA and proteins according to their molecular size. i. Analyze the characteristics of DNA molecule that affect the separation results in the gel electrophoresis. ii. Examine three common factors that are possibly affect the performance of gel electrophoresis.QUESTION 8 Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing. O A. You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing. O B. Whole genome sequencing files will have more reads in them because genomes are bigger than 165 amplicons. O C. The read alignment and contig assembly steps would probably be harder for whole genome sequencing. O D. The 165 molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer. O E. NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency. O F. Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.QUESTION 11 A graduate student, whom had not taken BIOL 531, localized the epitope for an antibody generated by the COVID19 vaccine to the peptide sequence below. S21P2 PSKPSKRSFIEDLLFNKV The student did a Blast search of the human proteome and found matches to human proteins. They are concerned that this means the vaccine could cause an autoimmune rection to human proteins. Is this student justified in this concern? Use statistics from a Blast search to justify your answer (Limit 5-6 sentences).
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