Question Which of the following activities of DNA pol lis MOST important in proofreading A 5' to 3' exonuclease B polymerase activity ligation activity 3' to 5' exonuclease

Q: Which activity of E. coli DNA polymerase I is responsible for proofreading the newly synthesized…

A: The first known polymerase is the DNA Polymerase I. It is the enzyme that participates in…

Q: Which of these is not a tool for comparing DNA sequences?   BLAST Fasta PLINK A dotplot e.g.…

A: The amount of data generated by next-generation sequencing platforms is enormous. The information…

Q: In ONE sentence define the function of the following 1. SSBS = The SSBS are single 2. Beta subunit…

A: These are all the enzymes used in replication of DNA Replication of DNA is a very important process…

Q: Conventional ligation procedures make use of enzymes with which activity?

A: DNA repair is a cellular mechanism that corrects various types of DNA damage that can occur due to…

Q: Which of these is not a tool for comparing DNA sequences? PLINK Fasta BLAST A dotplot e.g. dotlet

A: DNA polymerase is an enzyme. A primer is a single-stranded DNA fragment that binds to template DNA…

Q: What is the importance of the 3' to5' exonucleasevactivity of DNA polymerase

A: Enzymes serve as biological catalysts that fasten the rate of reaction by lowering the activation…

Q: DNA Replication and Protein Synthesis Review mmg Template strand SSB B- DNA polymerase Replication…

A: DNA replication is the cellular procedure for making an exact copy of its DNA. It's like making a…

Q: Multiple choice DNA polymerases can only elongate from:  -Free 3’ hydroxyl groups -The area ahead…

A: DNA polymerase is an enzyme that catalyzes the synthesis of DNA molecule. This enzyme is essential…

Q: Why is the following statement fals? RNA polymerase is a dangerous enzyme to the cell because it…

A: RNA polymerase is an essential enzyme that catalyzes the transcription of DNA into RNA. It is a…

Q: Eukaryotic DNA polymerases do not have 5'-3' exonuclease activity. _______ removes primers in…

A: Exonuclease activity is called the proofreading or editing function and it helps to repair the gaps…

Q: why DNA polymerase cannot remove all the RNA primers from Okazaki fragments to form a lagging…

A: Replication is the process by which cells create copies of their genetic material, DNA. DNA…

Q: QUESTION 1 The sequence of a DNA including the gene that you want to clone into a plasmid vector.…

A: Ans: The primers are set of oligo-nucleotide sequence used to initiate the synthesis or replication…

Q: During plasmid extraction, after the addition of acetate-containing neutralization buffer the large…

A: Plasmid extraction is a method for the isolation of DNA. In this method, purified plasmids are…

Q: Some of our DNA polymerases have a proofreading function. What is meant by this and how common is…

A: The classical deoxyribonucleic acid polymerases serve to duplicate all the deoxyribonucleic acid in…

Q: Single strand as a template plus 3' end to start DNA synthesis но- Polymerase works, DNA synthesis…

A: Addition of bases to the primer occurs as DNA replication proceeds :-

Q: optons to all 3 drop down menus are: 1. bottom strand 2. right to left 3. both right to left and…

A: DNA replication is the process in which one DNA can form copies of it. The enzymes involved during…

Q: DNA ligase

A: The correct option is Option 3. DNA ligase

Q: Restriction mapping of the delta chromosome I need help with question two please

A: the mean size is close to 4000 bp. DNA fragments of this length are useful in the lab, since…

Q: DNA polymerase functions from 5’ to 3’ direction. Explain this sentence. You

A: The DNA has been unraveled and unzipped. The helical structure has been unraveled. Special chemicals…

Q: is it possible that pcr primers could bind and amplify a sequence of dna that is difference from the…

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: To relax a supercoiled DNA, you may use, Select one: O a. DNA topoisomerase I O b. DNA helicase O c.…

A: Ans:- (a) DNA topoisomerase I

Q: "Semi conservative" replication means that after duplication of the DNA, one of the strands of DNA…

A: Replication of DNA is a biological process that belongs to the synthetic phase (S- phase) of the…

Q: You are using these two plasmids to make a recombinant DNA molecule. You want to make a molecule,…

A: Recombinant technology includes the process in which we can introduce our gene of interest with a…

Q: During plasmid extraction, after the addition of acetate-conta in ing neutralization buffer the…

A: Plasmids are extrachromosomal DNA in the bacterial cells. They are double stranded circular DNA,…

Q: DNA ladder 1 DNA ladder 2 bp ng/Sul 10000 8000 40 40 6000 3000 40 4000 40 700 600 3000 40 500 -2000…

A: Agarose gel electrophoresis is used to separate DNA molecules.

Q: Select all that are true about DNA gel electrophoresis O This technique can be used to separate DNA…

A: The gel electrophoresis is routinely used technique in molecular biology laboratories that helps in…

Q: 1)explain why it is far less important for RNA Polymerase to have proofreading activity than it is…

A: Fidelity of replication is the most important for the very existence of an organism. Besides its…

Q: How are the conformational changes of the infectious protein with respect to the normal one. Could…

A: Prions are abnormal, pathogenic agents which can be transmitted to induce abnormal folding of…

Q: Put the following DNA replication events in the correct order: Primase synthesizes an RNA primer.…

A: DNA replication is the process by which new DNA is synthesized from the old DNA template by…

Q: Why are the first 20 bases typically ignored from a Sanger sequencing ab1 file? O The data quality…

A: The most commonly used tool for detecting SNVs is Sanger sequencing, it involves DNA polymerase…

Q: 1b: Do DNA fragments move towards the positive or negative end of the gel box? Explain your answer.…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: A cell experiences a mutation where the DNA polymerase III enzyme can now add nucleotides to either…

A: A "nucleic acid" is a linear polymer of nucleotides that is a component of the cell's information…

Q: What are the answers to this

A: DNA replication is a process of producing another duplicate copy of the double strand DNA, where…

Q: Mismatch repair

A: DNA Repair: DNA repair is defined as the system of cellular responses which are associated with the…

Q: e. Identify the maximum number of amino acids in a polypeptide chain produced by a DNA containing…

A: DNA also known as deoxyribonucleic acid, is the molecule that houses the genetic instructions…

Q: Which of the mechanisms efficiently and effectively organize the DNA into a usable form?…

A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around one…

Q: What would you need to do to preform a successful PCR but only had thermolabile DNA polymerase? Can…

A: Polymerase chain reaction(in - vitro) technique is used for amplifying defined sequences of target…

Q: Holds the processive enzyme in prokaryotes Start of replication in prokaryotes Holds the processive…

A: 1. SSBP helicase holds processive enzymes in prokaryotes 2. Replication starts at ORI C ORIGIN OF…