### Enzyme Kinetics Problem Set#### Question #2The \( K_m \) of an enzyme is 5.0 mM.##### 2A. Calculate the substrate concentration when this enzyme operates at 10% of its maximum rate.*Answer: ______________*##### 2B. List the fraction of \( V_{max} \) that would be obtained when [S] (substrate concentration) = 0.1* \( K_m \), 0.5* \( K_m \), 2* \( K_m \), 10* \( K_m \).*Answer:- When [S] = 0.1 * \( K_m \) = ________- When [S] = 0.5 * \( K_m \) = ________- When [S] = 2 * \( K_m \) = ________- When [S] = 10 * \( K_m \) = ________*

Kinetics is the scientific discipline that studies reaction rates. Enzyme kinetics investigates the…

Answered: Question # 2 The Km of an enzyme is 5.0…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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QUESTION 19 Which enzyme (Enzyme 1, circle; Enzyme 2 square) has the worst affinity for the substrate? 5.0 4.0 EnzGraph 3.0 8 2.0 1.0 0.0 10 15 20 25 30 35 40 45 50 substrate (mM) Enz1 Enz2 A. Enzyme 1 (circles) B. Enzyme 2 (squares) C. cannot tell with information given D. both have the same affinity
Question 1: When the CAC is run in reverse by microorganisms that use it to fix carbon, the citrate synthase reaction is different and is catalyzed by an enzyme called ATP-citrate lyase. Write the reaction catalyzed by ATP-citrate lyase, then briefly (in one sentence) explain why the use of different chemistry (different from the ‘normal’ direction) makes sense here.
QUESTION 52 An organism is discovered that thrives in both the presence and absence of oxygen in the air. Curiously, the consumption of sugar increases as oxygen is removed from the organism's environment, even though the organism does not gain much weight. This organism O a. is photosynthetic O b. is a normal member of the animal kingdom O c. must utilize a molecule other than oxygen to accept electrons from the electron transport chain O d. only performs fermentation to make ATP
Question 31 True/False Question: Side chain of a serine residue in the active site of an enzyme can serve as a nucleophile during the catalysis. OTrue O False
QUESTION 18 Which of the following reactions is energetically favorable? The formation of peptide bonds O The addition of a phosphate.group to ADP O The breaking of glycosidic bonds O The formation of phosphodiester bonds
QUESTION 31 Four competitive inhibitors of an enzyme were found to exhibit the following K¡ values. Which is the worst inhibitor? K = 7x 10-5 M K1 = 3 x 10-6 M KI = 1 x 10-4 M K1 = 6 x 10-8 M QUESTION 32 What is the Km for a Michaelis enzyme if the 1/concentration of substrate intercept is -5.73e Mon a Lineweaver Burk plot of its reaction kinetics? Give your answer in 3 sigfīgs in scientific notation, for example, 1.52 e QUESTION 33 What is the charge of an ionized peptide in the mass spectrometer if its isotopic peaks are 0.20 m/z apart? O 6+ O 5+ wers to save all answers. stly cloud
QUESTION 20 Which enzyme (Enzyme 1, circle; Enzyme 2 square) has the best affinity for the substrate? 5.0 4.0 3.0 EnzGraph 8 2.0 1.0 0.0 10 15 20 25 30 35 40 45 50 substrate (mM) Enz1 Enz2 A. both have the same affinity B. Enzyme 2 (squares) C. cannot tell with information given D. Enzyme 1 (circles)
Question 1 a. What enzyme works on glucose-6-P when the energy charge is 0.80? b. What enzyme works on glucose-6-P when the energy charge is 0.97? c. What enzyme works on glycogen when the energy charge is 0.80?d. d. What enzyme works on glycogen when the energy charge is 0.97? e. What enzyme works on pyruvate when the energy charge is 0.80 (assume high O2)? f. What enzyme works on pyruvate when the energy charge is 0.97?
Question #3 Complex III (cytochrome bc₁ complex) in the electron transport chain transfers electrons from reduced coenzyme Q to cytochrome c. 3A. Write a balanced equation for the overall redox reaction performed by complex III. Indicate the electron donor and acceptor in the redox reaction. [Do not include the intermediates in this overall reaction. Do not account for proton pumping]. 3B. Calculate the change in the standard reduction potential for this reaction. [see table on page 2]. 3C Which has a lower amount of free energy at the standard state, the products or the reactants?
Question #12 An actively respiring bacterial culture is supplied with acetyl-CoA labeled on the methyl carbon with carbon- 14 (carbon-14 is radioactive). Shortly after adding the label the citric acid intermediates are isolated and the position of the carbon-14 atom is determined. 12A Where is the carbon-14 label on oxaloacetate after two turns of the citric acid cycle? Consider only the initial incorporation of carbon-14 in the first pass of labeled pyruvate through the citric acid cycle. relevant structures that show how pyruvate is transformed to alpha-ketoglutarate. No mechanisms are required, but provide balanced equations. You do not need to draw the structures of cofactors] draw the 12B. Suppose both of the acetyl-CoA acetate carbons were carbon-14 labeled. When does the first decarboxylation producing a radioactive 14CO2 occur?
Question #2: Consider the following data for Cell G run in 1 L chemostat, with a feed containing 30 g/L substrate: Feedrate (ml/hr) (c) Calculate 27.5 24.2 22.1 18.8 Steady State Cell Mass Concentration (g/L) 12.0 14.7 15.8 16.7 Steady State Substrate Concentration (g/L) 10.0 5.56 (d) Using the calculated kinetic parameters, 3.70 (a) Determine the kinetic parameters for growth (b) At each dilution rate, calculate Yx/s. Is it constant with dilution rate and substrate concentration? 2.23 (i) the critical dilution rate, D. (ii) the dilution rate corresponding to maximum productivity, Dm (iii) the maximum cell productivity (cells/L/hr) (i) Generate a plot of X and S vs D for a feed concentration of 25 g/L. (ii) What is the critical dilution rate for this condition? (iii) What is the dilution rate corresponding to maximum productivity at this condition? (iv) What is the maximum cell productivity at this condition? (v) For a reactor operating at this maximum productivity, what would be the…
QUESTION 8 The Bohr effect refers to the decrease in affinity of Hb for 02 when the pH goes up. A. the decrease in affinity of Hb for 02 when the pH goes down. B. the increase in affinity of Hb for O2 when the 02 concentration goes up. C. the decrease in affinity of Hb for O2 when the BPG concentration goes up. OD. the decrease in affinity of Hb for O2 when the BPG concentration goes down. OUESTI ON 9

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Question # 2 The Km of an enzyme is 5.0 mM. 2A Calculate the substrate concentration when this enzyme operates at 10% of its maximum rate. 2B List the fraction of Vmax that would be obtained when [S] (substrate concentration) = 0.1*Km, 0.5*Km, 2*Km, 10*Km

Question # 2 The Km of an enzyme is 5.0 mM. 2A Calculate the substrate concentration when this enzyme operates at 10% of its maximum rate. 2B List the fraction of Vmax that would be obtained when [S] (substrate concentration) = 0.1Km, 0.5Km, 2Km, 10Km