Question # 2 The Km of an enzyme is 5.0 mM. 2A Calculate the substrate concentration when this enzyme operates at 10% of its maximum rate. 2B List the fraction of Vmax that would be obtained when [S] (substrate concentration) = 0.1*Km, 0.5*Km, 2*Km, 10*Km

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Chapter1: Biochemistry: An Evolving Science
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### Enzyme Kinetics Problem Set

#### Question #2
The \( K_m \) of an enzyme is 5.0 mM.

##### 2A. Calculate the substrate concentration when this enzyme operates at 10% of its maximum rate.

*Answer: ______________*

##### 2B. List the fraction of \( V_{max} \) that would be obtained when [S] (substrate concentration) = 0.1* \( K_m \), 0.5* \( K_m \), 2* \( K_m \), 10* \( K_m \).

*Answer:
- When [S] = 0.1 * \( K_m \) = ________
- When [S] = 0.5 * \( K_m \) = ________
- When [S] = 2 * \( K_m \) = ________
- When [S] = 10 * \( K_m \) = ________*
Transcribed Image Text:### Enzyme Kinetics Problem Set #### Question #2 The \( K_m \) of an enzyme is 5.0 mM. ##### 2A. Calculate the substrate concentration when this enzyme operates at 10% of its maximum rate. *Answer: ______________* ##### 2B. List the fraction of \( V_{max} \) that would be obtained when [S] (substrate concentration) = 0.1* \( K_m \), 0.5* \( K_m \), 2* \( K_m \), 10* \( K_m \). *Answer: - When [S] = 0.1 * \( K_m \) = ________ - When [S] = 0.5 * \( K_m \) = ________ - When [S] = 2 * \( K_m \) = ________ - When [S] = 10 * \( K_m \) = ________*
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