Q2 An enzyme-catalyzed reaction has a Km-0.145 mM. Upon the use of a competitive inhibitor, the apparent Km=0.290 mM. The value of a rounded to the nearest whole number is: Given that 0.4 mM of inhibitor was added to the reaction, what is the Ki of the inhibitor in mM, rounded to one significant digit (e.g. 0.5):
Q2 An enzyme-catalyzed reaction has a Km-0.145 mM. Upon the use of a competitive inhibitor, the apparent Km=0.290 mM. The value of a rounded to the nearest whole number is: Given that 0.4 mM of inhibitor was added to the reaction, what is the Ki of the inhibitor in mM, rounded to one significant digit (e.g. 0.5):
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![Q2
An enzyme-catalyzed reaction has a Km-0.145 mM. Upon the use of a competitive inhibitor, the apparent
Km 0.290 mM. The value of a rounded to the nearest whole number is:
Given that 0.4 mM of
inhibitor was added to the reaction, what is the Ki of the inhibitor in mM, rounded to one significant digit
(e.g. 0.5):](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F572821e0-12d8-44f1-88bb-7fc88dda2af7%2F5881a49a-38c7-4e9d-966f-87fc8408bf18%2Ff9elswk_processed.png&w=3840&q=75)
Transcribed Image Text:Q2
An enzyme-catalyzed reaction has a Km-0.145 mM. Upon the use of a competitive inhibitor, the apparent
Km 0.290 mM. The value of a rounded to the nearest whole number is:
Given that 0.4 mM of
inhibitor was added to the reaction, what is the Ki of the inhibitor in mM, rounded to one significant digit
(e.g. 0.5):
![You are trying to confirm that an enzyme you are studying has the following amino acid in its active site,
and that this amino is critical for catalysis:
HS
OH
NH₂
Which of the following inhibitors could you use to provide evidence of the importance of this amino acid
for enzyme function?
Nevirapine
lodoacetate
2,3-Butanedione
Tetranitromethane
Diethylpyrocarbonate](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F572821e0-12d8-44f1-88bb-7fc88dda2af7%2F5881a49a-38c7-4e9d-966f-87fc8408bf18%2F5dfpbu7_processed.png&w=3840&q=75)
Transcribed Image Text:You are trying to confirm that an enzyme you are studying has the following amino acid in its active site,
and that this amino is critical for catalysis:
HS
OH
NH₂
Which of the following inhibitors could you use to provide evidence of the importance of this amino acid
for enzyme function?
Nevirapine
lodoacetate
2,3-Butanedione
Tetranitromethane
Diethylpyrocarbonate
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