Q1. If you add 25 g of food in 225 ml water, after mixing well, 1 ml is taken and added to 10 ml of water then a dilution of 1:9 (ratio 1 to 9) is made then 5 ml is added to 50 ml of water. Finally, 1 ml from this last dilution is plated. After incubation, 143 colonies are counted. What is the total count per g of the original sample? Show your calculations in details Answer:

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Q1. If you add 25 g of food in 225 ml water, after mixing well, 1 ml is taken and added to
10 ml of water then a dilution of 1:9 (ratio 1 to 9) is made then 5 ml is added to 50 ml of
water. Finally, 1 ml from this last dilution is plated. After incubation, 143 colonies are
counted. What is the total count per g of the original sample? Show your calculations in
details
Answer:
Transcribed Image Text:Q1. If you add 25 g of food in 225 ml water, after mixing well, 1 ml is taken and added to 10 ml of water then a dilution of 1:9 (ratio 1 to 9) is made then 5 ml is added to 50 ml of water. Finally, 1 ml from this last dilution is plated. After incubation, 143 colonies are counted. What is the total count per g of the original sample? Show your calculations in details Answer:
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