PRE-LAB ASSIGNMENT #2: CUMULATIVE PERCENT CHANGE AND RATE PRACTICE PROBLEMS To be completed before beginning week two of the Osmosis and the Scientific Method Lab. 1. The following data were collected during a study of the effect of potassium chloride (KCI) solute concentrations on osmosis in a potato core over a period of 60 minutes. Table 1: Mass (grams) of potato cores in different concentrations of KCI 0 Minutes 20 Minutes 0% KCI 1% KCI 4% KCI 0% KCI 1% KCI 4% KCI 6.2 Cumulative % Change 6.5 6.3 For 0% KCl at 0 min: a. Use the data in Table 1 and the formula for percent change in mass to complete Table 2 below. The first two calculations have been done for you as examples. Table 2: Cumulative percent change in mass of potato cores in different concentrations of KCl WE WWE MWEN 20 Minutes 40 Minutes 60 Minutes - www 0 Minutes 0 6.3 6.6 6.1 6.2-6.2 6.2 For 0% KCl at 40 minutes: 40 Minutes 60 Minutes Mass (current time point)- Mass (time point zero) Mass (time point zero) X 100 = 0% 6.5 6.6 5.8 For 0% KCI at 20 min: Complete the calculations for 1% and 4% KCI. 6.3-6.2 6.2 6.7 6.8 5.6 X 100 For 0% KCl at 60 minutes: X 100 = 1.6%
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
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