Based on your results, which chemotherapeutic agent(s) tested were broad-spectrum? Narrow-spectrum?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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LABORATORY REPORT FORM
EXERCISE 27
ANTIBIOTIC SENSITIVITY TESTING: THE KIRBY-BAUER PROCEDURE
What is the purpose of this exercise?
To determine the sensitivity or resistance of pathogenic aerobic and
compounds.
Facultative anaerobic bacteria to various antimicrobial
1. Complete the following chart:
CHEMOTHERAPEUTIC
AGENT
DISK
CODE
Streptomycin 10
Neomycin
Doxydycline
30
Chloramphenicol 30
10
10
2.
Ampicillin
Gentamicin
Bacitracin
10
Tetracycline 30
Staphylococcus aureus
S, I, or R
Zone
(mm)
15
30 25
3D
32
42
20
15
30
Escherichia coli
Zone
(mm)
28
20
28
19
30
0
S, I, or R
Pseudomonas
aeruginosa
Zone
(mm)
10
0
0
0
0
20
0
10
S, I, or R
Based on your results, which chemotherapeutic agent(s) tested were broad-spectrum? Narrow-spectrum?
3. Were any colonies growing within any of your zones of inhibition? If so, how would you determine if it
was a contaminant or a resistant colony?
Transcribed Image Text:LABORATORY REPORT FORM EXERCISE 27 ANTIBIOTIC SENSITIVITY TESTING: THE KIRBY-BAUER PROCEDURE What is the purpose of this exercise? To determine the sensitivity or resistance of pathogenic aerobic and compounds. Facultative anaerobic bacteria to various antimicrobial 1. Complete the following chart: CHEMOTHERAPEUTIC AGENT DISK CODE Streptomycin 10 Neomycin Doxydycline 30 Chloramphenicol 30 10 10 2. Ampicillin Gentamicin Bacitracin 10 Tetracycline 30 Staphylococcus aureus S, I, or R Zone (mm) 15 30 25 3D 32 42 20 15 30 Escherichia coli Zone (mm) 28 20 28 19 30 0 S, I, or R Pseudomonas aeruginosa Zone (mm) 10 0 0 0 0 20 0 10 S, I, or R Based on your results, which chemotherapeutic agent(s) tested were broad-spectrum? Narrow-spectrum? 3. Were any colonies growing within any of your zones of inhibition? If so, how would you determine if it was a contaminant or a resistant colony?
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