Questions: For the first two questions, refer to this photo of a SDS PAGE of ladder and antibody. The lane on the left (1) shows a protein ladder and the lane on the right (2) shows antibody. Question 1a. How many different proteins are in the protein ladder? 1b. Ladder and antibody samples are loaded into different the gel. 1c. The approximate size (in kDa) of "Heavy Chain" is: 1d. The approximate size (in kDa) of "Light Chain" is: on Answer 188- 93- 38- 28- 18- 14- kDa 6- 3- 1 2 -heavy chain -light chain
Questions: For the first two questions, refer to this photo of a SDS PAGE of ladder and antibody. The lane on the left (1) shows a protein ladder and the lane on the right (2) shows antibody. Question 1a. How many different proteins are in the protein ladder? 1b. Ladder and antibody samples are loaded into different the gel. 1c. The approximate size (in kDa) of "Heavy Chain" is: 1d. The approximate size (in kDa) of "Light Chain" is: on Answer 188- 93- 38- 28- 18- 14- kDa 6- 3- 1 2 -heavy chain -light chain
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Questions:
For the first two questions, refer to this photo of a
SDS PAGE of ladder and antibody.
The lane on the left (1) shows a protein ladder and the
lane on the right (2) shows antibody.
Question
1a. How many different proteins are in the protein ladder?
1b. Ladder and antibody samples are loaded into different
the gel.
1c. The approximate size (in kDa) of "Heavy Chain" is:
1d. The approximate size (in kDa) of "Light Chain" is:
on
Answer
188
kDa
93-
38-
28-
18-
14
6-
3-
12
-heavy chain
-light chain
Expert Solution
Step 1
1. Answer and explanation - 9 type protein are present on gel. 9 protein bands are present so 9 protein are exits.
2.ladder and antibody samples are loaded into different chromatography on gel.
If antibody digest only marceptoetheno or red using agents heavy and light two fragments are present. And two bands occur.
3 answer - the appropriate size of heavy chain = 49 kDa.
4. Answer - light chain size - appropriate
25 kDa.
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