please make sure final answer has correct sig figs Bovine serum albumin (BSA) has a molecular weight of 66.4 kDa and has an extinction coefficient of ?1% = 6.6. Calculate the concentration of BSA in solution if a sample has an absorbance of 1.35. The pathlength for the cuvette is 1 cm.
please make sure final answer has correct sig figs
Bovine serum albumin (BSA) has a molecular weight of 66.4 kDa and has an extinction coefficient of ?1% = 6.6. Calculate the concentration of BSA in solution if a sample has an absorbance of 1.35.
The pathlength for the cuvette is 1 cm.
In this problem, we are asked to calculate the concentration of Bovine Serum Albumin (BSA) in solution given its molecular weight and extinction coefficient, as well as the absorbance of a sample of the solution. The Beer-Lambert Law is used to relate the absorbance of the sample to the concentration of BSA, and we are provided with the necessary parameters to perform the calculation. The final answer must have the correct number of significant figures.
Step by step
Solved in 2 steps