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Q2 instructions: a. Which gel shows the successful trial? For the unsuccessful trials, what problem could have occurred? In all trials: the first lane is the ladder, the next 4 lanes are control reactions, and the last 2 lanes are experimental reactions. Hard mode: try to answer a few without looking at the hints Regular mode: see the list of possible mistakes on the last page of this document b. What is the genotype of the 2 unknown individuals? Trial 1 Trial 3 Trial 5 250 200 150 100 50 250 200 150 100 50 250 200 150 100 50 |||| Ladder Hh Oo HH OO || || || ||| hh oo I No DNA Unknown 2 Unknown 1 | || I Ladder ||||| Hh Oo HH OO hh oo || H = 150bp, h=85bp 0=100bp, o = 225bp Unknown 1 No DNA || || Unknown 2 || || Trial 2 Trial 4 Trial 6

Q2 instructions: a. Which gel shows the successful trial? For the unsuccessful trials, what problem could have occurred? In all trials: the first lane is the ladder, the next 4 lanes are control reactions, and the last 2 lanes are experimental reactions. Hard mode: try to answer a few without looking at the hints Regular mode: see the list of possible mistakes on the last page of this document b. What is the genotype of the 2 unknown individuals? Trial 1 Trial 3 Trial 5 250 200 150 100 50 250 200 150 100 50 250 200 150 100 50 |||| Ladder Hh Oo HH OO || || || ||| hh oo I No DNA Unknown 2 Unknown 1 | || I Ladder ||||| Hh Oo HH OO hh oo || H = 150bp, h=85bp 0=100bp, o = 225bp Unknown 1 No DNA || || Unknown 2 || || Trial 2 Trial 4 Trial 6

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Options for Q 2a a. This is the successful trial b.Cross contamination of template DNA c. HERC2 primers don't bind to template d.OCA2 primers don't bind to template e. One primer pair is generating a non-specific band f. Taq polymerase is non-functional g. Gel was run for too long h. Gel was not run long enough i. UV dye not added to the gel
Transcribed Image Text:Options for Q 2a a. This is the successful trial b.Cross contamination of template DNA c. HERC2 primers don't bind to template d.OCA2 primers don't bind to template e. One primer pair is generating a non-specific band f. Taq polymerase is non-functional g. Gel was run for too long h. Gel was not run long enough i. UV dye not added to the gel
Q2 instructions: a. Which gel shows the successful trial? For the unsuccessful trials, what problem could have occurred? In all trials: the first lane is the ladder, the next 4 lanes are control reactions, and the last 2 lanes are experimental reactions. Hard mode: try to answer a few without looking at the hints Regular mode: see the list of possible mistakes on the last page of this document b. What is the genotype of the 2 unknown individuals? 250 hh oo 200 150 Hh Oo HH 00 Unknown 2 No DNA Unknown 1 Unknown 2 hh ơơ 100 50 250 200 150 100 50 250 200 150 100 50 H = 150bp, h = 85bp 0 = 100bp, o = 225bp Trial 1 Ladder Trial 3 Trial 5 PO 4H 00 HH No DNA I UMOuxun Ladder || I || || || | || Trial 2 I| | | Trial 4 Trial 6
Transcribed Image Text:Q2 instructions: a. Which gel shows the successful trial? For the unsuccessful trials, what problem could have occurred? In all trials: the first lane is the ladder, the next 4 lanes are control reactions, and the last 2 lanes are experimental reactions. Hard mode: try to answer a few without looking at the hints Regular mode: see the list of possible mistakes on the last page of this document b. What is the genotype of the 2 unknown individuals? 250 hh oo 200 150 Hh Oo HH 00 Unknown 2 No DNA Unknown 1 Unknown 2 hh ơơ 100 50 250 200 150 100 50 250 200 150 100 50 H = 150bp, h = 85bp 0 = 100bp, o = 225bp Trial 1 Ladder Trial 3 Trial 5 PO 4H 00 HH No DNA I UMOuxun Ladder || I || || || | || Trial 2 I| | | Trial 4 Trial 6
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