Need help with question 5 and 6 Exercise II4. Complete the following table to calculate what volume of primers to add to your PCR master mix:Per SampleMaster MixAdded toReagent1X3.5XMaster Mix?Water19.9 ul69.7 µlBuffer (10X with Mg2+)2.5 μι8.8 μForward Primer (M13F), 10 µM1 ul3.5Reverse Primer (M13R), 10 µM1 ul3.5DNTPS (10 mM each, 40 mM total)0.5 ul1.8 μιTaq Polymerase0.1 pl0.4 ulTotal Volume25 μι87.5 µl50Have your Lab Instructor check your calculations before you add any reagents to your master mix.W Lab Instructor Initials:5. Suppose that you made a mistake when you set up the thermocycler program and omitted thefirst 2 minutes at 95°C. How would this impact your PCR?6. Suppose that you made a mistake when you set up the thermocycler program and onlyprogrammed for 10 cycles instead of 30 cycles. How would this impact your PCR?

Answer: 5 : the first 2 minutes at 95 degree Celsius is needed to open the dna if this is omitted…

5. Suppose that you made a mistake when you set up the thermocycler program and omitted the first 2 minutes at 95°C. How would this impact your PCR? 5. Suppose that you made a mistake when you set up the thermocycler program and only programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?

5. Suppose that you made a mistake when you set up the thermocycler program and omitted the first 2 minutes at 95°C. How would this impact your PCR? 5. Suppose that you made a mistake when you set up the thermocycler program and only programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter14: Biotechnology And Society

Section: Chapter Questions

Problem 12QP: DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that...

See similar textbooks

Related questions

Q: Since you have already learned the principal components and functions of the cell membrane, the…

A: “Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for…

Q: Describe the 6 anatomical changes that occur during a contraction to the sarcomere. 1. 2. 3. 4. 5.…

A: Hello, Thank you for posting questions. We follow company rules of answering one question at a time…

Q: I am a bit confused on why you switched the correct and incorrect statements. Could you clarify?

A: In genetics there are two types of alleles that are in charge of imparting the phenotypic effects on…

Q: The words with the commas in between them at the bottom of question 5 are the answer choices for…

A: It is the joining together of DNA molecules from two different species .

Q: Four cars of different masses are moving on the same road at the same speed. The mass of each car is…

A: Answer: Option is Car C

Q: 外 13 A ING 11

A: The given diagram is of the base of skull.

Q: Question 9 Erection is caused by O Enertic Nervous System O Parasympathetic reflex O Somatic…

A: The nervous system is a complex network of nerves and cells that carry messages to and from the…

Q: Part B: Preparation of Standard Graph & Determination of Concentration of Original Unknown Indigo…

A: Standard curves are plotted by using the light absorbance value and the solution concentration.…

Q: 1. A severe illness that may affect menstruating women after using tampons is abbreviated as: a. TSS…

A: 1. TSS - Toxic Shock syndrome is a life-threatening illness associated with the usage of tampons.…

Concept explainers

Immune Disorders

The collaboration between the cells and proteins that work together as a defense system against infection comprises the immune system.Each of them performs a variety of tasks to recognize and/or react against a foreign body.

Question

Need help with question 5 and 6

Exercise II
4. Complete the following table to calculate what volume of primers to add to your PCR master mix:
Per Sample
Master Mix
Added to
Reagent
1X
3.5X
Master Mix?
Water
19.9 ul
69.7 µl
Buffer (10X with Mg2+)
2.5 μι
8.8 μ
Forward Primer (M13F), 10 µM
1 ul
3.5
Reverse Primer (M13R), 10 µM
1 ul
3.5
DNTPS (10 mM each, 40 mM total)
0.5 ul
1.8 μι
Taq Polymerase
0.1 pl
0.4 ul
Total Volume
25 μι
87.5 µl
50
Have your Lab Instructor check your calculations before you add any reagents to your master mix.
W Lab Instructor Initials:
5. Suppose that you made a mistake when you set up the thermocycler program and omitted the
first 2 minutes at 95°C. How would this impact your PCR?
6. Suppose that you made a mistake when you set up the thermocycler program and only
programmed for 10 cycles instead of 30 cycles. How would this impact your PCR?

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicase
Cloned Libraries You are running a PCR to generate copies of a fragment of the cystic fibrosis (CF) gene. Beginning with two copies at the start, how much of an amplification of this fragment will be present after six cycles in the PCR machine?
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Provide a brief summary of the Sanger sequencing method.
DNA Profiles as Tools for Identification STRs are: a. used for DNA profiles b. repeated sequences present in the human genome c. highly variable in copy number d. all of these e. none of these
Genetics in Practice case studies are critical-thinking exercises that allow you to apply your new knowledge of human genetics to real-life problems. Case study Michelle was a 42-year-old woman who had declined counselling and amniocentesis at 16 weeks of pregnancy but was referred for genetic counseling after an abnormal ultrasound at 20 weeks of gestation. After the ultrasound, a number of findings suggested a possible chromosome abnormality in the fetus. The ultrasound showed swelling under the skin at the back of the fetuss neck; shortness of the femur, humerus, and ear length; and underdevelopment of the middle section of the fifth finger. Michelles physician performed an amniocentesis and referred her to the genetics program. Michelle and her husband did not want genetic counseling before receiving the results of the cytogenetic analysis. This was Michelles third pregnancy; she and her husband, Mike, had a 6-year-old daughter and a 3-year-old son. At their next session, the counselor informed the couple that the results revealed trisomy 21, explored their understanding of Down syndrome, and elicited their experiences with people with disabilities. She also reviewed the clinical concerns revealed by the ultrasound and associated anomalies (mild to severe intellectual disability, cardiac defects, and kidney problems). The options available to the couple were outlined. They were provided with a booklet written for parents making choices after the prenatal diagnosis of Down syndrome. After a week of careful deliberation with their family, friends, and clergy, they elected to terminate the pregnancy. Should physicians discourage a 42-year-old woman from having children because of an increased chance of a chromosomal abnormality?
Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA ligase _____ cDNA synthesis c. reverse transcriptase _____ DNA sequencing d. restriction enzyme _____ pasting DNA e. DNA polymerase (not Taq)
What Are Clones? Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone.
Why were radioactive sulfur and phosphorous used to label bacteriophage in Hershey and Chase’s experiments?
Why is so much effort being poured into genome mapping applications?
RECALL What vectors can be used for cloning?
Semiconservative or Conservative DNA Replication If 15N-Iabeled E. coli DNA has a density of 1.724 g/mL, 14N-labeled DNA has a density of 1.710 g/mL, and E. coli cells grown for many generations on 14NH4+as a nitrogen source are transferred to media containing 15NH4+as the sole N-source, (a) What will be the density of the DNA after one generation, assuming replication is semiconservative? (b) Suppose replication took place by a conservative mechanism in which the parental strands remained together and the two progeny strands were paired. Design an experiment that could distinguish between semiconservative and conservative modes of replication.