Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Question 13. After electroporating 20 ng of your plasmid into bacterial cells and recovering
the cells for 45 minutes at 37°C, you made a 1:100 dilution of the cell culture.
you plated 100 μl on a medium supplemented with IPTG, X-gal, and
ampicillin. The next morning you counted 150 white and 50 blue colonies on
your plate. How many of the transformants contain your DNA insert?
a.
15 colonies.
b. 200 colonies.
c. 50 colonies.
Question 14. Calculate the transformation efficiency per ng of the plasmid in the above
question (Question 13).
a. 1 x10³/ng.
b.
1 x10%/ng.
c.
d.
e.
1 x10%/ng.
1 x107ng.
1 x10%/ng.
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