**Transcription:**Phosphofructokinase (**PFK**) is the key regulatory enzyme of glycolysis. An allosteric inhibitor of PFK is ATP. An allosteric activator is AMP (and low ATP concentrations in the cell). Draw the plot of the reaction velocity vs. fructose-6-phosphate substrate concentration for both the high ATP cell concentrations and low ATP/high AMP concentrations.**Explanation of Required Diagram:**The graph to be drawn for this description would be a plot with the following characteristics:- **X-axis:** Fructose-6-phosphate substrate concentration- **Y-axis:** Reaction velocityThe plot should include two curves:1. **Curve for high ATP concentrations:** - This curve will typically show a lower reaction velocity at similar substrate concentrations compared to the low ATP/high AMP concentration. - It demonstrates the inhibitory effect of ATP on the enzyme activity.2. **Curve for low ATP/high AMP concentrations:** - This curve should show a higher reaction velocity, indicating the activation effect of AMP and low ATP levels on PFK activity.Both curves will likely exhibit a sigmoidal shape due to the allosteric nature of PFK, which does not follow simple Michaelis-Menten kinetics.

Glycolysis is a major metabolic pathway which carry out the breakdown of glucose to form pyruvate…

Answered: Phosphofructokinase (PFK) is the key…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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In glycolysis, the KM value of hexoquinase is 0.04 mM. At physiological conditions, the cellular concentration of glucose is between 4 mM to 15 mM. According with this information, which of the following statements is true (Choose all that are correct) The enzyme has a low affinity for its substrate The enzme is working at its maximum velocity The phosphorilation of glucose is very slow The small value of KM assures that glycolysis will continue even when the concentration of glucose drops below 4 mM. The hexoquinase is saturated with glucose
The following Michaelis-Menten plot shows the response of phosphofructokinase-1 (PFK-1), an enzyme in the glycolytic pathway, to the presence of ATP and AMP. Based on the data below, which of the following statements apply? Select all that apply. No inhibitors (low (ATP)) 1 mM ATP + 0.1 mM AMP 1 mM ATP 1.0 2.0 (Fructose-6-phosphate) mM Fig. : Regulation of PFK activity AMP is an allosteric modifier that enhances the binding of the substrate fructose 6-phosphate а. O b. AMP is an allosteric modifier that reduces the binding of the substrate fructose 6-phosphate ATP is an allosteric modifier that reduces the binding of the substrate fructose 6-phosphate O c. O d. ATP is an allosteric modifier that enhances the binding of the substrate fructose 6-phosphate Phosphofructokinase activity
Two reactions are shown below. These reactions are directly coupled in cells by an enzyme called hexokinase, which is a key enzyme in glycolysis. It is generally the case that reactions that are directly coupled by enzymes share some common reactants. What is the net reaction when these two reactions are combined? Glucose + Inorganic Phosphate + ATP + Water → Glucose 6-phosphate + ADP Glucose + ATP + Water → Glucose 6-phosphate + ADP + Inorganic Phosphate Glucose + ATP → Glucose 6-phosphate + Inorganic Phosphate Glucose + ATP → Glucose 6-phosphate + ADP
GAP + Pi + NAD+ ⟹1,3-BPG + NADH (Reaction 1) 1,3-BPG + ADP ⟹ 3-PG + ATP (Reaction 2) Which of the following statements concerning the information above is true? Select all that apply. A.Reaction 2 is an example of substrate level phosphorylation B.These reactions are written in a direction that would indicate gluconeogenesis is occurring in the liver C.The linking of Reaction 1 and Reaction 2 by the intermediate 1,3-BPG is an example of coupling of reactions D.Reaction 1 shows a redox reaction where the carbon skeleton is oxidized to generate electrons for a soluble electron carrier E.These reactions are irreversible under cellular conditions
Which of the following statements are true about the relationships of [S], KM, and Vmax? (Choose all that are true) As the [S] is increased, vo approaches the limiting value, Vmax KM = Vmax/2 The rate of product formed, vo, is at Vmax when [S] <<< KM KM and Vmax assist in finding the rate of the enzyme catalyzed reaction only if the reaction is considered irreversible.
Some enzymes have catalytic activity only limited by diffusion. Which rate constants of an enzyme- catalyzed reaction is/are rate limiting for the enzyme? How does this line up/compare to the rate limiting step of Michaelis-Menten Enzyme Kinetics? (Please show work and correct answer)
The following incorrect description of the HMS path is () A, 6-P-glucose can be converted into pentose phosphate through this pathway B. Four-carbon and seven-carbon sugars can be provided through this route C. For every mole of carbon dioxide produced when 6-P-glucose is converted to pentose phosphate, it also produces 1 mole of NADPH D, 6-P-glucose is decomposed in this way without consumption of ATP Among the following enzyme-catalyzed reactions that can generate substrate level phosphorylation to generate GTP are () A, hexokinase B, enolase C, succinate thiokinase D, succinate dehydrogenase The limiting factor of fatty acid synthesis in cell fluid is (). A, condensation enzyme B, hydration enzyme C, lipoacyl group transferase D, acetyl-CoA carboxylase Which of the following amino acids is an essential amino acid? () A.Thr B.Lys C.Met D.Arg
When ATP and xylose are added to hexokinase, the enzyme produces a small amount of xylose-5-phosphate along with a large amount of ADP. How does this observation support the induced fit model?
The AG of the reaction C6H12O6 + 60₂ --> 6CO₂ + 6H₂O is -686 kcal/mol glucose oxidized. The AG of the reaction ADP + P₁ --> ATP + H₂O is + 7.3 kcal/mol ATP synthesized. The oxidation of glucose can be coupled to the synthesis of ATP. If the coupling is 50% efficient, how many molecules of ATP can be synthesized per molecule of glucose oxidized? Round your answer to the nearest whole number.

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