Peptide mass determination. You have isolated a protein from the bacterium E. coli and seek to confirm its identity by trypsin digestion and mass spectrometry. Determination of the masses of several peptide fragments has enabled you to deduce the identity of the protein. However, there is a discrepancy with one of the peptide fragments, which you believe should have the sequence MLNSFK and an (M 1 H)1 value of 739.38. In your experiments, you repeat edly obtain an (M 1 H)1 value of 767.38. What is the cause of this discrepancy and what does it tell you about the region of the protein from which this peptide is derived?
Peptide mass determination. You have isolated a protein from the bacterium E. coli and seek to confirm its identity by trypsin digestion and mass spectrometry. Determination of the masses of several peptide fragments has enabled you to deduce the identity of the protein. However, there is a discrepancy with one of the peptide fragments, which you believe should have the sequence MLNSFK and an (M 1 H)1 value of 739.38. In your experiments, you repeat edly obtain an (M 1 H)1 value of 767.38. What is the cause of this discrepancy and what does it tell you about the region of the protein from which this peptide is derived?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Peptide mass determination. You have isolated a protein
from the bacterium E. coli and seek to confirm its identity
by trypsin digestion and mass spectrometry. Determination
of the masses of several peptide fragments has enabled you
to deduce the identity of the protein. However, there is a
discrepancy with one of the peptide fragments, which
you believe should have the sequence MLNSFK and an
(M 1 H)1 value of 739.38. In your experiments, you
repeat edly obtain an (M 1 H)1 value of 767.38. What is
the cause of this discrepancy and what does it tell you
about the region of the protein from which this peptide is
derived?
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