2. Carlo wanted to determine the amino acid sequence of BAYOKEM. The summary of the analysis performed on the peptide is shown below. Peptide BAYOKEM Number of amino acid residues Treatment/reagent/enzyme 9 total hydrolysis BAYO 4 KEM aminopeptidase trypsin total hydrolysis aminopeptidase pepsin total hydrolysis carboxypeptidase pepsin Based on the following results, answer the following questions: a. What is the N-terminal amino acid residue of peptide: Product(s) ala, arg, asp, cys, glu, his, lys, met, thr met+octapeptide BAYO + KEM ala, asp, arg, met met+tripeptide met+tripeptide cys, glu, his, lys, thr lys+tetrapeptide glu-his-lys+thr-cys (i) BAYO? (ii) KEM? (iii) BAYOKEM? b. Using the three-letter designations, what is the amino acid sequence of peptide (i) BAYO? (ii)KEM? (iii) BAYOKEM?
2. Carlo wanted to determine the amino acid sequence of BAYOKEM. The summary of the analysis performed on the peptide is shown below. Peptide BAYOKEM Number of amino acid residues Treatment/reagent/enzyme 9 total hydrolysis BAYO 4 KEM aminopeptidase trypsin total hydrolysis aminopeptidase pepsin total hydrolysis carboxypeptidase pepsin Based on the following results, answer the following questions: a. What is the N-terminal amino acid residue of peptide: Product(s) ala, arg, asp, cys, glu, his, lys, met, thr met+octapeptide BAYO + KEM ala, asp, arg, met met+tripeptide met+tripeptide cys, glu, his, lys, thr lys+tetrapeptide glu-his-lys+thr-cys (i) BAYO? (ii) KEM? (iii) BAYOKEM? b. Using the three-letter designations, what is the amino acid sequence of peptide (i) BAYO? (ii)KEM? (iii) BAYOKEM?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Peptide Sequencing
For number 2, show the OVERLAP method. (illustrate it please)
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