2. Carlo wanted to determine the amino acid sequence of BAYOKEM. The summary of the analysis performed on the peptide is shown below. Peptide BAYOKEM Number of amino acid residues Treatment/reagent/enzyme 9 total hydrolysis BAYO 4 KEM aminopeptidase trypsin total hydrolysis aminopeptidase pepsin total hydrolysis carboxypeptidase pepsin Based on the following results, answer the following questions: a. What is the N-terminal amino acid residue of peptide: Product(s) ala, arg, asp, cys, glu, his, lys, met, thr met+octapeptide BAYO + KEM ala, asp, arg, met met+tripeptide met+tripeptide cys, glu, his, lys, thr lys+tetrapeptide glu-his-lys+thr-cys (i) BAYO? (ii) KEM? (iii) BAYOKEM? b. Using the three-letter designations, what is the amino acid sequence of peptide (i) BAYO? (ii)KEM? (iii) BAYOKEM?

Biochemistry
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Chapter1: Biochemistry: An Evolving Science
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Peptide Sequencing

For number 2, show the OVERLAP method. (illustrate it please)

2. Carlo wanted to determine the amino acid sequence of BAYOKEM. The summary of the analysis
performed on the peptide is shown below.
Peptide
BAYOKEM
Number of amino acid
residues
Treatment/reagent/enzyme
9
total hydrolysis
BAYO
4
KEM
aminopeptidase
trypsin
total hydrolysis
aminopeptidase
pepsin
total hydrolysis
carboxypeptidase
pepsin
Based on the following results, answer the following questions:
a. What is the N-terminal amino acid residue of peptide:
Product(s)
ala, arg, asp, cys, glu,
his, lys, met, thr
met+octapeptide
BAYO + KEM
ala, asp, arg, met
met+tripeptide
met+tripeptide
cys, glu, his, lys, thr
lys+tetrapeptide
glu-his-lys+thr-cys
(i) BAYO?
(ii) KEM?
(iii) BAYOKEM?
b. Using the three-letter designations, what is the amino acid sequence of peptide
(i) BAYO?
(ii)KEM?
(iii) BAYOKEM?
Transcribed Image Text:2. Carlo wanted to determine the amino acid sequence of BAYOKEM. The summary of the analysis performed on the peptide is shown below. Peptide BAYOKEM Number of amino acid residues Treatment/reagent/enzyme 9 total hydrolysis BAYO 4 KEM aminopeptidase trypsin total hydrolysis aminopeptidase pepsin total hydrolysis carboxypeptidase pepsin Based on the following results, answer the following questions: a. What is the N-terminal amino acid residue of peptide: Product(s) ala, arg, asp, cys, glu, his, lys, met, thr met+octapeptide BAYO + KEM ala, asp, arg, met met+tripeptide met+tripeptide cys, glu, his, lys, thr lys+tetrapeptide glu-his-lys+thr-cys (i) BAYO? (ii) KEM? (iii) BAYOKEM? b. Using the three-letter designations, what is the amino acid sequence of peptide (i) BAYO? (ii)KEM? (iii) BAYOKEM?
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