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SDS-PAGE separates proteins based primarily on which of the following characteristics: O a. their intrinsic charges O b. their three-dimensional shapes C. their sizes in basepairs O d. their expression levels e. their molecular weights Of. their His-tags

SDS-PAGE separates proteins based primarily on which of the following characteristics: O a. their intrinsic charges O b. their three-dimensional shapes C. their sizes in basepairs O d. their expression levels e. their molecular weights Of. their His-tags

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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**SDS-PAGE separates proteins based primarily on which of the following characteristics:** - a. their intrinsic charges - b. their three-dimensional shapes - c. their sizes in base pairs - d. their expression levels - e. their molecular weights - f. their His-tags --- **Explanation:** SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) is a common laboratory technique used to separate proteins based on their molecular weights. In this method, SDS, an anionic detergent, denatures proteins and gives them a uniform negative charge. As a result, proteins are separated solely by size as they migrate through the polyacrylamide gel, with smaller proteins moving faster than larger ones. This technique is widely used for analyzing protein mixtures in research and clinical laboratories.
Transcribed Image Text:**SDS-PAGE separates proteins based primarily on which of the following characteristics:** - a. their intrinsic charges - b. their three-dimensional shapes - c. their sizes in base pairs - d. their expression levels - e. their molecular weights - f. their His-tags --- **Explanation:** SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) is a common laboratory technique used to separate proteins based on their molecular weights. In this method, SDS, an anionic detergent, denatures proteins and gives them a uniform negative charge. As a result, proteins are separated solely by size as they migrate through the polyacrylamide gel, with smaller proteins moving faster than larger ones. This technique is widely used for analyzing protein mixtures in research and clinical laboratories.
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