Once you had your DNA sample extracted from your cells, why did we add the yellow Master Mix solution? Specifically explain the roles of the following: a. primers b. Taq polymerase C. Mg (Why would we add this back in when earlier we wanted to remove Mg*?). d. individual nucleotides
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
![4. Once you had your DNA sample extracted from your cells, why did we add the yellow Master
Mix solution? Specifically explain the roles of the following:
a. primers
b. Taq polymerase
C. Mg (Why would we add this back in when earlier we wanted to remove Mgʻ*?).
d. individual nucleotides
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