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- Compare the reovirus genome to those of influenzavirus and bacteriophage MS2. Why do reovirusreplication events have to happen in the nucleocapsid?Assume you isolate a single stranded (+) RNA virus. When you examine the proteins in the virus, you find that it does NOT contain replicase enzymes within its capsid. Which of the following is true? This virus must have a gene that encodes replicase. This virus will not be able to enter a host cell. Its genome cannot be translated (the process of translation) by the host cell ribosomes. A DNA copy of the viral genome has to be made before viral genes are expressed. This virus must lack surface antigens.a. Mycobacteriophage genes occasionally begin with GUG or, even more rarely , UUG (rather than AUG ). Which amino acids corre spond to these codons ? b. Yeast genes occasionally begin with CUG, ACG , or AUC . Which amino acids correspond to these codons ?
- Why bacteriophage is called t4?The genes for both the α- and βglobin chains of hemoglobin contain introns (i.e., they are split genes). How would this fact affect your plans if you wanted to introduce the gene for α-globin into a bacterial plasmid and have the bacteria produce α-globin?A virus that has which type of genome must carry replicase within the viral particle? (choose all that apply) ds DNA ss (+) DNA ss (–) DNA ss (+) RNA ss (–) RNA Which viral type has a genome that can be directly translated? (choose all that apply) ds DNA ss (+) DNA ss (–) DNA ss (+) RNA ss (–) RNA
- 1) Where in the heck did Class I transposons originate? a DNA mutations. b Bacteria. c Prophages. d Retroviruses. 2) What do you think about humans only having about 22,500 genes but we contain about 100,000 proteins?! a The production of quaternary shape in proteins can contribute to protein variation. b That's the work of the spliceosome! c Post-translation modifications in the Golgi Apparatus are responsible for some of that. d All the answers are correct.Briefly describe the lytic and lysogenic cycles of bacteriophages.In your answer, explain what a prophage is?Bacteriophage T4 has a linear double-stranded DNA genome, yet mapping many mutations, as shown in Figure, generates a circular linkage map. How might you explain this discrepancy?
- 1. Below is a sequence of DNA bases. =T ACTICACGAGTGAGACT a) Transcribe to MRNA: AUGAAGUGCUCACUCUGA b) Translate to a sequence of amnio acids: Second Base Ala = Alanine Arg = Arginine Asn = Asparagine Asp - Aspartic acid Cys = Cysteine Gin = Glutamine Glu - Glutamic acid Gly-Glycine His Histidine lle - Isoleucine Leu -Leucine A G code amino acid code amino acid code amino acid code amino acid UUU UCU UAU UGU phe tyr cys UUC UCC UAC UGC C ser UUA UCA UAA STOP UGA STOP leu UUG UCG UAG STOP UGG trp G CUU CCU CAU CGU his CUC CC CÁC CGC C leu pro arg CUA CCA CAA CGA gin CUG CG CAG CGG G. Lys Lysine Met - Methlonine AUU ACU AAU AGU asn ser AUC ile ACC AAC AGC Phe- Phenylalanine Pro- Prolie Ser Serine thr AUA ACA AAA AGA lys arg AUG met ACG AAG AGG GUU GCU GAU GGU ThrThroonio hp fs 米 IOI 19 f10 11 12 ins Ins pr First Base Third BaseSome scientists are trying to engineer bacteriophageto treat bacterial infections in humans when theinfections do not respond to chemical antibiotics.a. What possible advantages might phage therapyhave over antibiotic therapy?b. Describe potential difficulties that would need tobe overcome for phage therapy to succeed.c. How might researchers best confront the issue thatbacterial cells could become resistant to bacteriophage just as they could to antibiotics?Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infection