a. The promoter is to the right so transcription goes right to left. b. This sequence 5’ AGCTACGGGGTG 3’ is a part of the template strand from the MIDDLE of gene. Write it in the middle of one of your lines. Use pencil in case of errors. c. Write the sequence of the coding strand of DNA on the other line. d. Indicate the start of transcription with an arrow like this and write “promoter” where it must be located. e. Draw a thicker (or different colored) line to indicate a strand of newly synthesized mRNA. Label 5’ and 3’ ends. By the template sequence on your diagram, write the sequence of mRNA that is produced from this template. f. In what ways are the sequences of the coding strand and the mRNA similar? g. In what ways are the coding strand and the mRNA different?
a. The promoter is to the right so transcription goes right to left.
b. This sequence 5’ AGCTACGGGGTG 3’ is a part of the template strand from the MIDDLE
of gene. Write it in the middle of one of your lines. Use pencil in case of errors. c. Write the sequence of the coding strand of DNA on the other line.
d. Indicate the start of transcription with an arrow like this and write “promoter” where it must be located.
e. Draw a thicker (or different colored) line to indicate a strand of newly synthesized mRNA. Label 5’ and 3’ ends. By the template sequence on your diagram, write the sequence of mRNA that is produced from this template.
f. In what ways are the sequences of the coding strand and the mRNA similar? g. In what ways are the coding strand and the mRNA different?
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Class 12 Transcription Worksheet
Work in teams but each student must complete and turn in their own worksheet
1. There can be thousands of genes along a DNA molecule. For a given gene, one strand of DNA is
known as the template strand and the other strand is known as the coding strand. The RNA is
complementary and anti-parallel to the DNA template strand.
a. The catalyst for transcription is called
RNA Polymerase
b. New RNA can only be made in the
3"
direction.
Explain why.
It can only add RNA nucleotides (A, U, C, G) to the end strand of 3".
2. In the space below, the two parallel lines indicate a section of double-stranded DNA that is
unwound in this region. (Only a small section is unwound at a time). Label the 5' and 3' ends.
5'
a. The promoter is to the right so transcription goes right to left.
b. This sequence
of gene. Write it in the middle of one of your lines. Use pencil in case of errors.
5' AGCTACGGGGTG 3'
is a part of the template strand from the MIDDLE
c. Write the sequence of the coding strand of DNA on the other line.
d. Indicate the start of transcription with an arrow like this
located.
and write "promoter" where it must be
e. Draw a thicker (or different colored) line to indicate a strand of newly synthesized mRNA. Label 5'
and 3' ends. By the template sequence on your diagram, write the sequence of mRNA that is
produced from this template.
f. In what ways are the sequences of the coding strand and the mRNA similar?
g. In what ways are the coding strand and the mRNA different?
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