Map of pUC18 is shown on the here. Describe how to select recombinant clones if a foreign DNA is inserted in to the polylinker site of pUC18 and then introduced into E. coli cells. Hindll Sphl Sbfl Pstl Acci Hincll Sall Xbal BamHI Aval Smal Xmal Acc651 Kpnl Banll Eco53kl Saci Apol ECORI lacz MCS LacR binding site Plac PUC18 Amp 2686 bps PMB1 ori

Q: What would three possible reasons be that a PCR wouldn't work after cloning a TAQ sequence into a…

A: Even though you've verified Taq protein expression through SDS-PAGE, your PCR might still fail for a…

Q: Consider the following chart: What type of mutation is this? DNA: TAC GCA TGG AAT MRNA: AUG CGU ACC…

A: By the translation process polypeptide chain is being synthesized from the mRNA sequence by the help…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: 3) mutated:3' TA C A CC TTG coA CGA CTA'S MRAA transcript: AuG uCG AACGCU Gcu GAu. amino acid:…

A: A mutation is a change in the DNA sequence of an organism. Mutations can result from errors in DNA…

Q: you carry out DNA editing using CRISPR whter the editing template DNA has strands labeled with heavy…

A: DNA (Deoxyribonucleic acid) is edited with the help of several molecular tools. CRISPR (Clustered…

Q: If the template DNA is 3' A GCTAC5' then the mRNA sequence is

A: Ans- As mentioned the DNA template 3’ – A G C T A C – 5’ The mRNA sequence will be – a)          5’…

Q: You are interested in the rapid mutation rate of the polymerase of a virus XY, that inserts its…

A: The mutation changes the amino acid at the third position. Specifically, it changed from Glycine…

Q: The DNA sequence below is used by the primase to synthesize a primer. What is the sequence of the…

A: The primer of this sequence will be complementary to it. The complementary bases are as follows: A…

Q: You plan to clone exon 11 of the HEXA gene (Section C) into the multiple cloning site (MCS) of the…

A: Answer well explained above

Q: This is a restriction map for the 250 base pair plasmid pSage. Restriction sites for the restriction…

A: Restrictions endonucleases are enzymes that cut down the DNA into fragments near or at the site of…

Q: The following illustrates a jagged double-strand DNA break resulting from Cas9 cleavage that…

A: "Investigating the results of DNA repair through non-homologous end joining (NHEJ) following Cas9…

Q: The schematic diagram below shows the functional organization of transcribing RNA polymerase. Match…

A: Transcription is the next step after replication in the central dogma of biology. It is the process…

Q: Describe what each of the 12 bands on your Western blot should have been. Remember, the 12 bands…

A: Western blotting: It is a technique used to identify and locate proteins based on their ability to…

Q: Below is a portion of an exon from a gene that encodes protein Y in the genome of the plant…

A: Ans 1 :  Yes, there is a mutation in third codon when counting from 5/ to 3/ on template strand in…

Q: EcoRI Sacl Kpnl Aval ampR Xmal Smal lacZ BamHI Xbal Sall Accl HinclI Pstl Sphl HindIII Bam H1 Bam H1…

A: Answer :- Option (C) is correct. - 1. :-

Q: None

A: Thes images is only for reference purposes

Q: a. Assume that replication has been initiated at that ORI. Provide the sequence of the primer that…

A: DNA is the genetic material of almost all living organisms. It contains information that is…

Q: During muscle contraction, some energy is supplied from creatine phosphate. Which of the following…

A: Muscle contraction occurs when tension-generating sites within muscle cells are activated.

Q: Match the following (most appropriate combinations): Gel filteration chromatography Electrophoresis…

A: 1) Gel filtration chromatography - separation of proteins in a column based on size. In this type of…

Q: 27. Given the following plasmid map, list how many fragments would exist and their bp length when…

A: Restriction enzymes are used by bacteria to cleave foreign DNA. They can then be isolated and used…

Q: For tailed PCR primers the "tails" can have sequence that is NOT complementary to the target…

A: PCR - (polymerase chain reaction) is used to amplify the DNA sequence through repetitive cycles. It…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: After characterizing the DNA composition of various cats, you identify a protein-coding gene in…

A: In this scenario, we aim to study the structure of the protein product STRIPES, derived from a gene…

Q: The beginning of the hexose kinase gene's sequence can be found below, the +1 nucleotide is…

A: Given:

Q: Identify one mistake that was introduced in the following figure.  True or false? The first few…

A: Since it is the method through which DNA is utilized as a template to produce RNA, transcription is…

Q: 2a. Assume that replication has been initiated at that ORI. Provide the sequence of the primer that…

A: AT GC base pairing: In double stranded DNA, the guanine (G) base on one strand can form three…

Q: Which of the following protospacer adjacent motif sequences would be recognized by Cas9?     GCG…

A: Cas9 stands for CRISPR (clustered regularly interspaced short palindromic repeats) associated…

Q: DNA containing the genes gin+ phe+ lys+ was used to transform bacterial cells of genotype gln- phe-…

A: As per the question :-  Genotypes and number of individuals obtained are as:-  A ) gly + phe+ lys +…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.