Map of pUC18 is shown on the here. Describe how to select recombinant clones if a foreign DNA is inserted in to the polylinker site of pUC18 and then introduced into E. coli cells. Hindll Sphl Sbfl Pstl Acci Hincll Sall Xbal BamHI Aval Smal Xmal Acc651 Kpnl Banll Eco53kl Saci Apol ECORI lacz MCS LacR binding site Plac PUC18 Amp 2686 bps PMB1 ori

Q: What would three possible reasons be that a PCR wouldn't work after cloning a TAQ sequence into a…

A: Even though you've verified Taq protein expression through SDS-PAGE, your PCR might still fail for a…

Q: 5' UTR OP This region is specific sequence surrounding the start codon. OK OS OT 5' and 3' UTR LEU2…

A: In bacteria, there is a small circular double-stranded extra-chromsomal DNA that can replicate…

Q: Describe the banding pattern seen in the gel. What does that tell you about the role of annealing…

A: Gel electrophoresis is a laboratory technique for separating charged molecules such as DNA, RNA, and…

Q: Which temperature is Taq polymerase's optimal temperature O 20 degrees C 72 degrees C 55 degrees C O…

A:

Q: You are working with a laboratory strain of E.coli that contains a 10 base-pair deletion in the Lac…

A: Introduction of the concept: The given concept is based on the lac operon in E.coli. The operon…

Q: EboV RNA from Guinea Pig EboV RNA from Guinea Pig GAU ACG UUC GUC AAU EboV DNA CTA TGC AAG CAG TTA…

A: Given sequence of EboV strain 1 is CTA TGC AAG CAG TTA and strian 2 is TCA TGT CAG CAA CTA.

Q: Using figure (a) above as guide, list the five components in the CRISPR-Cas9 bacterial defense…

A: CRISPR is a acronym for Clustered regularly interspaced short palindromic repeats. It forms a very…

Q: 5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and…

A: The production of RNA from DNA is known as transcription that occurs within the cytoplasm of…

Q: EboV RNA from Guinea Pig EboV RNA from Guinea Pig GAU ACG UUC GUC AAU EboV DNA CTA TGC AAG CAG TTA…

A: A missense mutation is a mistake in the DNA which results in the wrong amino acid being incorporated…

Q: The following illustrates a jagged double-strand DNA break resulting from Cas9 cleavage that…

A: "Investigating the results of DNA repair through non-homologous end joining (NHEJ) following Cas9…

Q: Site specific recombination using Cre-Lox system shown below will result in InxP JoxP Transformation…

A: Question - 1 - Site specific recombination using Cre-Lox system shown below will result in…

Q: Please make this as SIMPLE as possible Question: regarding to horizontal gene transfer

A: Horizontal gene transfer mainly occurs in prokaryotes.

Q: Here is a list of knockout gene mutations that are desired to make in E. coli genome. You should…

A: A number of knockout gene mutations in the E. coli genome are shown to us in this scenario. Each…

Q: Describe what each of the 12 bands on your Western blot should have been. Remember, the 12 bands…

A: Western blotting: It is a technique used to identify and locate proteins based on their ability to…

Q: From the following choices, select the DNA sequence that is classified as a mirror repeat. ATCATC O…

A: DNA or deoxyribonucleic acid is the genetic material in living organisms that is composed of…

Q: that best fits the phrase below (1-20) Cis-acting B Post-transcriptional modification…

A: Francis Crick proposed central dogma. The RNA is formed from DNA with the help of enzyme RNA…

Q: Below is a portion of an exon from a gene that encodes protein Y in the genome of the plant…

A: Ans 1 :  Yes, there is a mutation in third codon when counting from 5/ to 3/ on template strand in…

Q: a. Assume that replication has been initiated at that ORI. Provide the sequence of the primer that…

A: DNA is the genetic material of almost all living organisms. It contains information that is…

Q: How many restrictions sites are present in the EcoRI. Calculate the number of restriction sites for…

A: Restriction endonucleases or restriction enzymes are enzymes that cleave or cut nucleic acids at…

Q: 33) Judging by mosaic clone images above, what effect does loss of dmyc likely have on cell growth?…

A: Approach to solving the question:1. Observation of Mosaic Clone Images: Begin by carefully examining…

Q: Match the following (most appropriate combinations): Gel filteration chromatography Electrophoresis…

A: 1) Gel filtration chromatography - separation of proteins in a column based on size. In this type of…

Q: A) Tet-Off B) Tet-On rRTA ITA TRE Target Transgone TRE Target Transgene +Dox +Tc or +Dox tTA ETA TRE…

A: as the diagram shows the tet-on and tet-off system

Q: Parts A-C in the table show the genotypes for two partial diploid strains of the lac operon. Gene…

A: A group of genes called the lac operon are involved in the bacterial metabolism of lactose. *Three…

Q: Based on the following wild type sequence, indicate if each of the mutations should be classified as…

A: Mutations are changes in the sequence of DNA which may or may not affect the phenotype of the…

Q: For tailed PCR primers the "tails" can have sequence that is NOT complementary to the target…

A: PCR - (polymerase chain reaction) is used to amplify the DNA sequence through repetitive cycles. It…

Q: Based on the following wild type DNA sequence, indicate if each of the mutations should be…

A: A mutation is a change in a DNA sequence. Mutations can result from DNA copying mistakes made during…

Q: The 1000 aa protein (Protein X) you are studying has several putative caspase cleavage sites. You…

A: Western blotting is a molecular biology technique that is used to detect a specific protein from a…

Q: For the following sequence please design an 18 base pair forward primer.  ATG TCA AAA GCT GTC GGT…

A: Introduction :  Primers are short oligonucleotides that are chemically synthesised and complementary…

Q: i have a gene (3222bp) i want to put it into a expression vector (pet 28b+),when i did pcr i flanked…

A: Introduction  Gene cloning is the technique to produce the exact copies of the desired gene by…

Q: Please select all cellular processes that do NOT rely on RecA protein function as far as we…

A: RecA is a 38kDa recombinase protein found in prokaryotes and is essential for the maintenance,…

Q: please match the following with the right answer here are the option

A: Solution that destabilize the cell wall/ membrane of the bacteria :- Ice bath Protein that gives the…

Q: Which of the following protospacer adjacent motif sequences would be recognized by Cas9?     GCG…

A: Cas9 stands for CRISPR (clustered regularly interspaced short palindromic repeats) associated…

Q: explain this image for the ecoli bacteria mutation trpE

A: Introduction The trp operon is a type of operon system found in E. coli bacteria. The trp operon…

Q: What is the role of the following molecules in the CRISPR mechanism? *

A: CRISPR stands for clustered regularly interspaced short palindromic repeats. These are a set of DNA…

Q: Site specific recombination using Cre-Lox system shown below will result in JoxP foxP faaP Fed JoP

A: Cre-Lox system is a site specific recombination technology, which is used for the deletion,…

Q: 5' ATGATCTCGTAA 3' 3' TACTAGAGCATT 5'

A: DNA, or deoxyribonucleic acid, is a hereditary molecule that encodes the blueprint for the growth,…

Q: Construct a circular chromosome map and show the direction of the transfer and various locations of…

A: Bacterial conjugation is the transfer of genetic material (plasmid) from one bacteria to another in…

Q: a b A-T (FT169) Vector IR (10 Gy): WT Wortmannin: IR (10 Gy) cap T2609 «DNA-PKcs 1 2 3 1 2 3 4 Ku80…

A: Phosphorylation of a molecule is the process of attachment of phosphoryl group.

Q: 1. What is the total size of the PMBBS plasmid in bp? Answer: bp 2. How many cut sites on the pMBBS…

A: As per the guidelines, we are supposed to answer only three subparts. Kindly repost the question…

Q: ORIGIN 1 aagcttctcc accagcgctt tcagctgcgc cagggtctgc tgccggtcct ccgcggcggc 61 gacaggccag gccgactggc…

A: Introduction: Lac operon is a polycistronic structural gene that is regulated by a promoter and…

Q: Which area (A., B. C. or D) represents the region important for maintaining the gene of interest…

A: A vector is defined as a DNA molecule which can be used for carrying foreign DNA segment or gene of…

Q: 7 8 9 9 10 11 1 TONSL vs Primary log2FPKM Raw counts 2 foldchange log2FC PValue KTB_103_pr…

A: Method: Identification of Differentially Expressed Genes (DEGs) in Breast Cancer Using RNA-Seq and…

Q: O The lac operon in E.coli encodes enzymes necessary for the breakdown of lactose. For each enzyme…

A: There are two type of lac operon mutation, constitutive and uninducible lac mutation. LacI-…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.