Q: Refer to the DNA sequence provided: 3’ -TACTGAAGCGGCAGCCCCGCATGAGTAGACCTTACT-5’ a. What is the…
A: In this question, we are given anticoding sequence of DNA. This is also called as antisense strand…
Q: A terminator loop is… Group of answer choices a. A tertiary structure in DNA that causes DNA…
A: Introduction :- There are three main steps , that are present in any molecular events , like - in…
Q: Please select appropriate word in each bracket CRISPR-Cas9 is a system for directly changing the…
A: Genetic material is nothing but the sequence of nucleic acids which is called as DNA. It contains…
Q: The piRNA, miRNA and RNAi pathways are all similar and may have evolved from some early function of…
A: Both microRNA (miRNA) and RNA interference (RNAi) pathways has an enzyme called DICER(RNAse III…
Q: Can you select answers in each bracket? CRISPR-Cas9 is a system for directly changing the sequence…
A: CRISPR is a group of DNA sequences present in the genomes of bacteria and archaea, which are…
Q: Describe how RNA polymerase backtracking could function to increase the fidelity of transcription.
A: Transcription of the genetic information from DNA into RNA is the central process of gene…
Q: miRNAS P. are synthesized as long pri-miRNAs (up to 1000 nucleotides). Q. are transcribed by RNA…
A: Micro RNAs are found in animals, plants and some viruses. These are single stranded RNA molecule…
Q: Explain how the data of Fire and Mello suggested that doublestrandedRNA is responsible for the…
A: RNA (Ribo Nucleic Acid) is the genetic material similar to DNA (Deoxyribose Nucleic Acid) found in…
Q: Propose some specific uses of a modified CRISPR-Cas system as a general RNA-guided device for…
A: CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas is a gene-editing tool. It…
Q: Describe one effect of a Cas (CRISPR-Associated) protein on DNA molecules.
A: The (CRISPR) Cas system acts in a sequence-specific manner by recognizing and cleaning foreign DNA…
Q: What functional information about a genome can be determined through applications of chromatin…
A: Chromatin Immunoprecipitation (ChIP) is a type of technique based on immunoprecipitation and is…
Q: In the CRISPR-Cas system, does the tracrRNA act as a scaffold, guide, ribozyme, blocker, decoy,…
A: The CRISPR-Cas system is a defense system against foreign invaders like bacteriophage, transposons,…
Q: Explain the CRISPR-Cas Mechanism for RNA-Guided Destruction of Invading DNA ?
A: CRISPR or clustered regularly interspaced short palindromic repeats denotes a set of sequences of…
Q: xplain The mRNA codon of valine is: GUC UGG CCA TTG
A: Each codon in mRNA is made up of three nucleotides and represents a certain amino acid (hence, it is…
Q: One problem that researchers sometimes encounterwhen editing genomes with CRISPR/Cas9 is that oneor…
A: The gene therapy or gene editing is an asset for humans, as many of the gene-related disorders can…
Q: You were able to obtain the RNA of the influenza virus. If you injected this into a susceptible host…
A: The influenza viruses have segmented, negative-strand RNA genomes that require replication by an…
Q: Describe in detail the process of RNAI including the major protein involved. What is the role of…
A: Biotechnology is the field that makes use of biology to solve problems undergoing the making of some…
Q: Define about primary miRNAs (pri-miRNAs) ?
A: MicroRNAs (miRNAs) are small regulatory RNAs derived from stem-loop sections of original transcripts…
Q: miRNAs target endogenous mRNAs in a sequence-specific manner. Explain, conceptually, how one might…
A: To explain: How to identify potential mRNA targets for a given miRNA if only the sequence of the…
Q: Diagram the mechanism by which CRISPR-cas functions in the immune system of bacteria.
A: CRISPR (clustered regularly interspaced palindromic repeats) is a type of defense mechanism only for…
Q: What would a scientist need to use CRISPR-Cas system to introduce a specific point mutation into a…
A: When the target DNA is located, Cas9, one of the CRISPR system's enzymes, latches to it and chops…
Q: What is the actual biological purpose of the CRISPR/Cas9 system?
A: All biological information of an organism is stored inside the biomolecule called DNA. Gene are the…
Q: How does the flexibility feature facilitate the function of regulatory RNAs?
A: Regulatory RNA is defined as a non-coding RNA molecule that will further, play a role in processes…
Q: Define `transcriptome'. Briefly describe the genome tiling array technology to `visualize'…
A: Hi dear, here's your answer what you want.can you please give me a like for this answer. * Every one…
Q: s the PAM sequence required for Cas9-mediated cleavage of a single-stranded strand of DNA (when that…
A: The protospacer adjacent motif is a short DNA sequence that follows the DNA region targeted for…
Q: List three important properties of RNA polymerase from E. coli.
A: RNA polymerase from E.coli consists of 5 subunits- two alpha subunits, one beta subunit, one beta…
Q: Contrast the origins and functions of the three maincategories of small regulatory RNAs (miRNAs,…
A: The non-coding ribonucleic acid (RNA) that plays a role in the activation or inhibition in the…
Q: Imagine Cas9 used a 30 base RNA molecule instead of a 20 base molecule. How often will it cut? Hint:…
A: Compared to previous techniques for modifying DNA or RNA, this new approach is much faster and…
Q: Refer to the DNA sequence provided: 3’ -TACTGAAGCGGCAGCCCCGCATGAGTAGACCTTACT-5’ a. What is the mRNA…
A: Gene expression is the process by which the instructions in our DNA are converted into a functional…
Q: explain about CRISPR - CAS 9 system .
A: CRISPR means clustered regularly interspersed short palindromic repeats. CAS 9 means CRISPR…
Q: Question: how does the flu without the track arise and what does it mean male type splicing if the…
A: Flu first arrise from birds and animals then it transmitted into humans. It's spread from virus.…
Q: Q. Here is the sequence of a portion of a bacterial gene. The template strand is on the bottom:…
A: Since you have posted a question with multiple sub-parts, we will solve first three sub- parts for…
Q: BIOLOGICAL IMPLICATIONS OF CRISPR-CAS9
A: Cells are the littlest units of life, and subsequently are regularly alluded to as the "building…
Q: Predict the replication step for the novel Miovirus. This virus was determined to have a dsRNA…
A: Introduction A virus is a small piece of genetic material, such as DNA or RNA, encased in a protein…
Q: b. Explain how Nirenberg and Leder/Matthaei were able to create many of the codon/amino acids found…
A: Nirenberg and P. Leder developed a triplet binding assay in 1964. It was an historically important…
Q: Match each factor involved in miRNA biogenesis with its function , pri-miRNA forms a hairpin that is…
A: RNAi (ribonucleic acid interference) is also known as gene silencing. It is a biological process…
Q: Compare and contrast anti-miRNA oligonucleotides, locked nucleicacids (LNAs), and antagomirs, which…
A: Cancer is also known as malignancy and it is a disease which involves abnormal growth of cells which…
Q: MIRNA (Ca he 'seed region').. 3: what actually happened if Splicing factor mutated? Give an example…
A: In spliceosomes during post transcriptional modification introns are removed from the m RNA which…
Q: Q2. Do miRNA and siRNA both derive from the same source? Explain.
A: The answer is- No
Q: Describe two ways that piRNA can suppress the activity of transposable elements
A: Transposon silencing is a form of transcriptional gene silencing targeting transposons.…
Q: How is miRNA-206 beneficial to an organism?
A: miRNA-206 : A microRNA is a small single-stranded non-coding RNA molecule found in plants, animals…
MAKE CONNECTIONS Compare the CRISPR-Cas system
to the miRNA system discussed in Concept 18.3, including
their mechanisms and their functions.
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- Describe how RBPs can prevent miRNAs from degrading an RNA molecule.Compare and contrast anti-miRNA oligonucleotides, locked nucleicacids (LNAs), and antagomirs, which may eventually be used to treat certain forms of cancer.What would be a possible result if a miRNA that perfectly complemented E3 ligase was successfully processed in the cell?
- What are the differences between miRNA and siRNA? (Select all that apply.) OmiRNAs carry the genetic information from the DNA in the nucleus directly to the cytoplasm, and siRNAs are involved in the degradation of specific mRNA molecules. miRNAs are from 22 to 30 bases long, and siRNAs are 22 bases long. O miRNAs carry the genetic information from the DNA in the nucleus directly to the cytoplasm, and siRNAs help with the processing of the initial mRNA transcribed from DNA into a mature form. miRNAs prevent translation of certain mRNAs, and siRNAs are involved in the degradation of specific mRNA molecules. miRNAs are 20 to 22 bases long, and siRNAs are from 20 to 30 bases long.You are a research scientist studying miRNA processing. You currently know everything about the pathway except for one detail: whether Dicer resides in the nucleus or in the cytoplasm. You have an experiment setup where you have a miRNA that completely complements the GFP (green fluorescent protein) gene in a model yeast cell. You plan to mutate Exportin 5 and make it dysfunctional, then you will inject synthetic pre-miRNA either in its double strand form or its hairpin single strand form into the cell. Should you inject the synthetic pre-miRNA into the nucleus or the cytoplasm? a) b) If you inject hairpin single stranded pre-miRNA into the appropriate location in the cell, what color do you expect the yeast to be if Dicer is in the nucleus? What color would the yeast be if Dicer is in the cytoplasm? Briefly explain your reasoning.Consider the following sequence fragment of an mRNA. Which of the miRNAS below would be competent for gene silencing? 5'-AUGCAAGCAUUGGCCAAGCUU-3' 5'-AUGCAAGCAUUGGCCAAGCUU-3 5'-UACGUUCGUAACCGGUUCGAA-3' 5'-AAGCUUGGUUAAUGCUUGCAU-3' 5'-UUCGAACCAAUUACGAACGUA-3' 3'-UUCGAACCAAUUACGAACGUA-5 3'-AUGCAAGCAUUGGCCAAGCUU-5'
- miRNAs target endogenous mRNAs in a sequence-specific manner. Explain, conceptually, how one might identify potential mRNA targets for a given miRNA if you only know the sequence of the miRNA and the sequence of all mRNAs in a cell or tissue of interest.What is the difference between crRNA and tracrRNA? Why are both needed for Cas9 to function? What does PAM stand for? Where is it found? What is the difference between Non-homologous End Joining (NHEJ) and Homology Directed Repair (HDR)?Create a hybrid cDNA-mRNA molecule using reverse transcriptase and oligo-dT primer, what is step 3 of making a cDNA library?
- . In many bacterial species, regulatory sRNAs havebeen identified by transcriptome sequencing (RNASeq). How do researchers know that the small RNAspecies identified by cDNA sequencing are regulatorysRNAs rather than fragments of longer mRNAs?Mechanistic analysis of silencing mechanisms showed that exogenous double-stranded RNA was processed into short fragments (short interfering RNAs or siRNAs) with a similar size to miRNAs. How do mRNAs represent a similar endogenous system to transgene silencing?E22. The method of Northern blotting is used to determine the amount and size of a particular RNA transcribed in a given cell type. Alternative splicing (discussed in Chapter 12) produces mRNAs of different lengths from the same gene. The Northern blot shown here was made using a DNA probe that is complementary to the MRNA encoded by a particular gene. The mRNA in lanes 1 through 4 was isolated from different cell types, and equal amounts of total cellular MRNA were added to each lane. 2 3 4 Lane 1: MRNA isolated from nerve cells Lane 2: MRNA isolated from kidney cells Lane 3: MRNA isolated from spleen cells Lane 4: MRNA isolated from muscle cells Explain these results. | |