. In many bacterial species, regulatory sRNAs havebeen identified by transcriptome sequencing (RNASeq). How do researchers know that the small RNAspecies identified by cDNA sequencing are regulatorysRNAs rather than fragments of longer mRNAs?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
. In many bacterial species, regulatory sRNAs have
been identified by transcriptome sequencing (RNASeq). How do researchers know that the small RNA
species identified by cDNA sequencing are regulatory
sRNAs rather than fragments of longer mRNAs?
Once the whole RNA is extracted and cDNA is synthesized, the cDNA is then sent for sequencing. Once the sequenced data comes the sequenced data is being compared with the available data bases of sRNA which can be done with different bioinformatic tool.Those short RNAs which are matching with the existing data are sRNA while others are just parts of long mRNAs.
- BSRD is a repository for published sRNA sequences with multiple annotations and expression profiles.
- SRD is a database of Staphylococcus aureus sRNAs with sequences, predicted structures, and genome start and end sites.
- sRNAdb is a databse of sRNAs from Gram-positive bacterial species with sequence annotation.
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