Magnesium chloride is an important in the PCR experiment becauseit adds chloride to the buffer solutionO it makes the phosphate group gain a positive charge) it helps stabilize the DNA backboneit makes the deoxyribose group gain a positive chargeWhich experiment requires the use of detergents?isolation of DNAPCR experimentGel electrophoresisEffect of temperature on digestion and fermentation Arrange the following steps in the experiment regarding the effect oftemperature on digestion of starch by amylase41.Pipette starchsolution in yourtubesPrepare thewater bath atdifferenttemperaturesUse jodine asindicator of areactionRepeat withyour amylasesolutionPlace the tubesin your steambathArrange the following steps in the gel electrophoresis experiment4Wait for thegel to turnopaquePour theagaroseMake sure thegel box is onan evensurfacePlace thecomb near thenegativeelectrodeHook up theelectrodes

PCR (Polymerase Chain Reaction) is a technique which is used to make millions or billions of copies…

Answered: Magnesium chloride is an important in…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

In an analysis of the number of different bases in a DNA sample, which result would be consistent with the base-pairing rules? * A+T>G+C O AC+T
If we extract DNA of a pool of cells, will that impact the DNA sequencing? In other words, having more than one cell will affect the DNA sequence of our sample? Same as 1, but with RNA. Cite one problem you expect when investigating a pool of cells.
Which one of the following is true of DNA polymerase, based on our coverage? The 2'OH of the dNTP H-bonds to the palm region to position the dNTP near the primer. It has at least one metal ion that serves as a bridge' between the enzyme and the substrate. O The Mg2 is a strong nucleophile. It has a double-displacement mechanism. The attacker in the mechanism of action is considered to be the alpha-phosphate of the dNTP
Synthesis of hybrid genes can occur by Directed mutagenesis O Using overlap primers O RT-PCR O Inverse PCR
Please help I am stuck, I have provided everything needed
Which of these is not a tool for comparing DNA sequences?
In polymerase chain reaction, which of the following is NOTa purpose of the primers you added to the the PCR tube? O To denature the DNA To give DNA polymerase a molecule to add nucleotides to To target our sequence of interest To bind to template DNA on either side of the sequence we want to amplify
You start running your gel and after a few minutes notice that the colored bands from the loading dye are moving in the wrong direction. This is happening because you accidentally: O Forgot to add loading dye to your DNA samples O Forgot to add ethidium bromide to your DNA samples O Attached the negative electrode to the gel box on the end where samples were loaded Attached the positive electrode to the gel box on the end where samples were loaded
Which of the following enzymes has 5' to 3' exonuclease activity? DNA polymerase lII O Telomerase DNA ligase O Primase DNA polymerase l
After you design the PCR primers, you run the PCR on fluid from the patient’s nasal swab. Next, you need to evaluate the results. PCR makes billions of copies of just one sequence in a sample. Since you know the sequence, you also know the length (number of nucleotides) of the region you copied. There are about 130 nucleotides in the N gene fragment. This is typically stated as 130 base pairs. How can you visualize DNA and estimate its size? Load the DNA sample into an agarose gel (similar in consistency to jello) and apply an electric current to the gel. The DNA is charged and will move through the gel. The longer the DNA fragment, the more slowly it moves. The DNA is visualized by adding a fluorescent dye to your sample that sticks to DNA. When you look at the gel under UV light, the DNA should glow. What is the charge of a DNA molecule? Based on #1, would you expect DNA to be drawn to the (+) or (-) electrode of the gel electrophoresis chamber?
Which of the strands of DNA could act as a PCR primer for the DNA sequence shown below? 5’-CCCTGGGCTCTGTAAATGTTTCTAAGTG-3’ 3’-GGGACCCGAGACATTTACAAAGATTCAC-5’ A. 3′-ACTGTTAGA-5′ B. 3′-AAATTTGGC-5′ C. 3′-ATGCTTTGA-5′ D. 5′-GGGACCCGA-3′ E. 5′-CCCTGGGCT-3′
I need help with a biology lab question, a DNA isolation lab Cold temperatures were used to cause the DNA to flocculate (form larger comples). What was the purpose of adding isoporpyl alcohol to the mixture? Hint: DNA dissolves in water and undissolves in alcohol.

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Magnesium chloride is an important in the PCR experiment because O it adds chloride to the buffer solution O it makes the phosphate group gain a positive charge it helps stabilize the DNA backbone it makes the deoxyribose group gain a positive charge