Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 5 1/[S], (L/mol)

Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 5 1/[S], (L/mol)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
4. Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 1/[S], (L/mol) 5
Transcribed Image Text:4. Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 1/[S], (L/mol) 5
Expert Solution
Step 1

The line weaver burk plot is a double reciprocal plot, i.e., it is the graph for 1V vs 1[S],

where V is the enzyme velocity, and [S] is the substrate concentration. 

 

The linear curve given fits the equation: y=4.20x+18.3, where X represents 1[S] and Y represents 1V,

 

steps

Step by step

Solved in 3 steps with 1 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS