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Lalculate: You need a digestion buffer consisting of the following: 15 mM NaCI 75 mM Tris-CI, pH 8 16 mM EDTA, pH 8 0.8% sodium dodecyl sulphate U NWU° 83 0.75 mg/mL proteinase K You are supplied with the following: NaCI (Mr = 58.443 g/mol) 2.5 M Tris-CI, pH 8 solution (1 litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10% sodium dodecyl sulphate solution Proteinase K solution (50 mg dissolved in 1 ml dd.H20) How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations.

Lalculate: You need a digestion buffer consisting of the following: 15 mM NaCI 75 mM Tris-CI, pH 8 16 mM EDTA, pH 8 0.8% sodium dodecyl sulphate U NWU° 83 0.75 mg/mL proteinase K You are supplied with the following: NaCI (Mr = 58.443 g/mol) 2.5 M Tris-CI, pH 8 solution (1 litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10% sodium dodecyl sulphate solution Proteinase K solution (50 mg dissolved in 1 ml dd.H20) How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Lalculate: You need a digestion buffer consisting of the following: 15 mM NaCI 75 mM Tris-CI, pH 8 16 mM EDTA, pH 8 0.8% sodium dodecyl sulphate O NWU° 83 0.75 mg/mL proteinase K You are supplied with the following: NacI (Mr = 58.443 g/mol) * 2.5 M Tris-CI, pH 8 solution (1 litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10% sodium dodecyl sulphate solution Proteinase K solution (50 mg dissolved in 1 mL dd.H20) How will you prepare 500 mL of the digestion buffer? Show all your steps * and calculations.
Transcribed Image Text:Lalculate: You need a digestion buffer consisting of the following: 15 mM NaCI 75 mM Tris-CI, pH 8 16 mM EDTA, pH 8 0.8% sodium dodecyl sulphate O NWU° 83 0.75 mg/mL proteinase K You are supplied with the following: NacI (Mr = 58.443 g/mol) * 2.5 M Tris-CI, pH 8 solution (1 litre) EDTA, sodium salt (Mr = 380.2 g/mol) 10% sodium dodecyl sulphate solution Proteinase K solution (50 mg dissolved in 1 mL dd.H20) How will you prepare 500 mL of the digestion buffer? Show all your steps * and calculations.
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