Lab 10- PCR For the following equipment, know what it looks like, its name, and its function: micropipette, tip, water bath, centrifuge, thermal cycler For the following solutions, know its name and its function: saline, InstaGene, MasterMix What's the difference between pv92 and Alu? On what chromosome is pv93 located? What are the names of and general functions of the 3 steps to PCR (denature, anneal, extend)? What is the relevance of the 2 different stop points of the plunger on a micropipette? What was the importance of primers and Taq? What was the cofactor we tried to take away from DNase yet later gave to Taq?

Q: Kpnl 4. Plasmid Z has a size of 7 kb, and the map shows Kpnl (K) and Pstl (P) cut sites relative to…

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Q: Why is this technique used?

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A: Bacteria is the unicellular organisms they are different types of bacteria many lives in different…

Q: PCR employs in vitro DNA replication. DNA replication in vivo-i.e., in a cell, normally requires…

A: Polymerase chain reaction (PCR) is a method widely used to make many copies of a specific DNA…

Q: Genetic Mutations and Tests Across 3 The second phase of a PCR reaction where the primers bind to…

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A: The polymerase chain reaction is the laboratory technique, utilized for making multiple copies of a…

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Q: Genetics Question 3

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Q: 2. To tube 1, add 10 μl of 50:50 AT:GC DNA (tube B). 3. Add 5 μl of Buffer to tubes 2-7. 4. Remove 5…

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Q: 1. Calculate the size of the resulting fragments as they will occur after digestion and write the…

A: Hello. Since your question has multiple parts, we will solve the first question for you. If you want…

Q: Pls help me complete the below sentences asap!!

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Q: What type of label is used for fluorescence in situ hybridization (FISH) probes?

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Q: Give typed full explanation  you made a recombinant plasmid (it is circular) that contains the…

A: Here, the correct answer is option 1) The plasmid will be cut once, making a linear DNA fragment.

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Q: 2. Restriction Enzyme Mapping - use any resources to assist you including the hints below. A…

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Q: Why DNA methylation needed for DNA replication ? * Your answer What is the general way for…

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Q: The difference between PCR and real-time PCR is that real-time PCR: can determine the DNA sequence,…

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Virus

Viruses are infectious microscopic agents that can only reproduce in live cells of animals, plants, or microbes and are smaller than bacteria. Vertebrates, invertebrates, plants, protists, and fungi are among their many hosts. Viruses are unable to multiply on their own due to the lack of necessary cellular machinery, but they can guide the cell machinery of a host to make additional viruses by infection. The virus enters and attaches to a particular host cell, injects its genetic material, and reproduces by using the host’s genetic material. Viruses cause infection by taking entry into the host cell and using the host cell’s enzymes and raw materials to multiply.