Lab 10- PCR For the following equipment, know what it looks like, its name, and its function: micropipette, tip, water bath, centrifuge, thermal cycler For the following solutions, know its name and its function: saline, InstaGene, MasterMix What's the difference between pv92 and Alu? On what chromosome is pv93 located? What are the names of and general functions of the 3 steps to PCR (denature, anneal, extend)? What is the relevance of the 2 different stop points of the plunger on a micropipette? What was the importance of primers and Taq? What was the cofactor we tried to take away from DNase yet later gave to Taq?

Q: QUESTION 1 The sequence of a DNA including the gene that you want to clone into a plasmid vector.…

A: Ans: The primers are set of oligo-nucleotide sequence used to initiate the synthesis or replication…

Q: please solve this step by step with clear explaination

A: To convert the given amounts of DNA from micrograms (μg) to microliters (μl), we need to use the…

Q: 1. Explain what primers are and what purpose they serve in a PCR reaction. Explain the main steps…

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Band, Band Size, and what bands corresponds?

A: The image shows gel electrophoresis containing DNA fragments of various sizes. Gel electrophoresis…

Q: How many RT-PCR products generated from 13 copies of mRNA (= cDNA) from 33 PCR amplification cycles?…

A: RT-PCR Reverse transcription-polymerase chain reaction, or RT-PCR, is presently the most sensitive…

Q: Bonus: Which PCR process below generates an almost "instant signal" of a template in a sample using…

A: Polymerase chain reaction (PCR) is a method widely used to make many copies of a specific DNA…

Q: These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and…

A: These are the results after running Agarose Gel Electrophoresis of the cut/uncut pUC19 plasmid and…

Q: During gel electrophoresis, in what direction (toward which electrode) does DNA move toward?…

A: There are different molecular biology tools and techniques used to analyze and quantify…

Q: After transduction we move to mutagenesis analysis. Using the transduction plate，PCR I found that…

A: In this problem, we are analyzing the results of a mutagenesis experiment involving several genes.…

Q: What needs to happen so the reporter molecule can emit fluorescence that is not quenched?

A: Real time PCR is a technique used to monitor and quantify the PCR product reaction in real time. The…

Q: This is what the LAB FLOW would look like:                                   CULTURE BACTERIA…

A: PCR means polymerase chain reaction. Polymerase chain reaction is a method widely used to rapidly…

Q: The following statements are true about common gene cloning procedures except: -DNA plasmids can…

A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…

Q: An optimum ligation reaction should contain approximately 50 ng of vector. Given your concentrations…

A: It is given that an optimum ligation reaction should contain approximately 50 ng of the vector. We…

Q: Why should a student use the SQ3R method?

A: In this question it is asking that why should a student use SQ3R method.

Q: In a test tube, a particular DNA polymerase has an error rate of 1 mistake in every 106 bases…

A: Any change in the sequence of DNA or the structure and number of chromosomes in an organism is…

Virus

Viruses are infectious microscopic agents that can only reproduce in live cells of animals, plants, or microbes and are smaller than bacteria. Vertebrates, invertebrates, plants, protists, and fungi are among their many hosts. Viruses are unable to multiply on their own due to the lack of necessary cellular machinery, but they can guide the cell machinery of a host to make additional viruses by infection. The virus enters and attaches to a particular host cell, injects its genetic material, and reproduces by using the host’s genetic material. Viruses cause infection by taking entry into the host cell and using the host cell’s enzymes and raw materials to multiply.