Kinetic assays were performed to better understand the activity of the enzyme 245C in the absense and presence of molecule B. The enzyme follows Michaelis-Menten kinetics. a) Calculate the VO for this enzyme when [S]=0.03mM and no molecule B is present. b) Using a properly labelled Lineweaver-Burke plot with accurate x- and y-intercepts, describe the effect of molecule B on the enzyme. Briefly explain how molecule B could be causing these effects
Catalysis and Enzymatic Reactions
Catalysis is the kind of chemical reaction in which the rate (speed) of a reaction is enhanced by the catalyst which is not consumed during the process of reaction and afterward it is removed when the catalyst is not used to make up the impurity in the product. The enzymatic reaction is the reaction that is catalyzed via enzymes.
Lock And Key Model
The lock-and-key model is used to describe the catalytic enzyme activity, based on the interaction between enzyme and substrate. This model considers the lock as an enzyme and the key as a substrate to explain this model. The concept of how a unique distinct key only can have the access to open a particular lock resembles how the specific substrate can only fit into the particular active site of the enzyme. This is significant in understanding the intermolecular interaction between proteins and plays a vital role in drug interaction.
Kinetic assays were performed to better understand the activity of the enzyme 245C in the absense and presence of molecule B. The enzyme follows Michaelis-Menten kinetics.
a) Calculate the VO for this enzyme when [S]=0.03mM and no molecule B is present.
b) Using a properly labelled Lineweaver-Burke plot with accurate x- and y-intercepts, describe the effect of molecule B on the enzyme. Briefly explain how molecule B could be causing these effects.
![[S] mM
Vo (mM/min) E+S
0.4
Vo (mM/min) E+S+B
0.1
0.3
2.0
7.0
6.0
10.0
31.0
24.0
20.0
52.0
40.0
40.0
77.0
60.0
60.0
93.0
72.0
200.0
129.0
100.0
1000.0
149.0
115.0
2000.0
152.0
118.0
5000.0
154.0
120.0](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F63b21e12-32ab-4236-a3ec-7d0c88bb8aaa%2F2a99148e-5644-45ab-8949-64ad6e847059%2Fd6dtre_processed.png&w=3840&q=75)
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