The efficiency nof fluorescence resonance energy transfer (FRET) is given by: R$ = R + R° A solution is prepared containing linear molecules, each consisting of an anthracene donor and a tryptophyl peptide acceptor separated by n L-prolyl linker units. The solution absorbs 0.230 mJ of laser radiation with a wavelength of 273 nm and emits 1.35 ×10² mJ of fluorescence at a wavelength of 293 nm. Given that R, = 2.20 nm, ø; = 0.36 for anthracene, and the approximate length of one linker %3D unit is 0.34 nm, determine n.

The efficiency nof fluorescence resonance energy transfer (FRET) is given by: R$ = R + R° A solution is prepared containing linear molecules, each consisting of an anthracene donor and a tryptophyl peptide acceptor separated by n L-prolyl linker units. The solution absorbs 0.230 mJ of laser radiation with a wavelength of 273 nm and emits 1.35 ×10² mJ of fluorescence at a wavelength of 293 nm. Given that R, = 2.20 nm, ø; = 0.36 for anthracene, and the approximate length of one linker %3D unit is 0.34 nm, determine n.

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter23: Enzymes

Section: Chapter Questions

Problem 23.4P: Compare the activation energy in uncatalyzed reactions and in enzyme-catalyzed reactions.

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Question

The efficiency n of fluorescence resonance energy transfer (FRET) is given by:
R + R°
A solution is prepared containing linear molecules, each consisting of an
anthracene donor and a tryptophyl peptide acceptor separated by n L-prolyl linker
units. The solution absorbs 0.230 mJ of laser radiation with a wavelength of 273
nm and emits 1.35 ×10² mJ of fluorescence at a wavelength of 293 nm. Given that
R.
= 2.20 nm, ø= 0.36 for anthracene, and the approximate length of one linker
unit is 0.34 nm, determine n.

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