The observed fluorescence lifetime in the absenceof a quencher is 1.4 ns. In the presence of a quencher thefluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the fluorescence quantum yieldsin the presence and absence of the quencher. ¢,/¢,_0
The observed fluorescence lifetime in the absenceof a quencher is 1.4 ns. In the presence of a quencher thefluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the fluorescence quantum yieldsin the presence and absence of the quencher. ¢,/¢,_0
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter15: Molecular Luminescence Spectrometry
Section: Chapter Questions
Problem 15.2QAP
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The observed fluorescence lifetime in the absence
of a quencher is 1.4 ns. In the presence of a quencher the
fluorescence lifetime is 0.8 ns. Calculate the quenching efficiency. which is the ratio of the fluorescence quantum yields
in the presence and absence of the quencher. ¢,/¢,_0
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