An amino acid on the surface of a protein was labelled covalently with 1.5-I AEDANS and another was labelled covalently with FITC. The fluorescence quantum yield of 1.5-I AEDANS decreased by 10 per cent due to quenching by FITC. What is the distance between the amino acids? (Refer to Table 17G.3 for the appropriate value of R0.) Table 17G.3 Values of R, for some donor-acceptor pairs*DonorAcсеptorR/nmNaphthaleneDansyl2.2DansylODR4.3PyreneCoumarin3.91.5-I AEDANSFITC4.9Tryptophan1.5-I AEDANS2.2TryptophanHaem (heme)2.9"Additional values may be found in J.R. Lacowicz, Principles of fluorescence spectros-copy, Kluwer Academic/Plenum, New York (1999).*Abbreviations:Dansyl: 5-dimethylamino-1-naphthalenesulfonic acidFITC: fluorescein 5-isothiocyanate1.5-I AEDANS: 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (3)ODR: octadecyl-rhodamine

When energy is transferred from an excited donor to an acceptor group through a non-radiative…

Answered: Table 17G.3 Values of R, for some…

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

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An amino acid on the surface of a protein was labelled covalently with 1.5-I AEDANS and another was labelled covalently with FITC. The fluorescence quantum yield of 1.5-I AEDANS decreased by 10 per cent due to quenching by FITC. What is the distance between the amino acids? (Refer to Table 17G.3 for the appropriate value of R₀.)

Table 17G.3 Values of R, for some donor-acceptor pairs*
Donor
Acсеptor
R/nm
Naphthalene
Dansyl
2.2
Dansyl
ODR
4.3
Pyrene
Coumarin
3.9
1.5-I AEDANS
FITC
4.9
Tryptophan
1.5-I AEDANS
2.2
Tryptophan
Haem (heme)
2.9
"Additional values may be found in J.R. Lacowicz, Principles of fluorescence spectros-
copy, Kluwer Academic/Plenum, New York (1999).
*Abbreviations:
Dansyl: 5-dimethylamino-1-naphthalenesulfonic acid
FITC: fluorescein 5-isothiocyanate
1.5-I AEDANS: 5-(((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (3)
ODR: octadecyl-rhodamine

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