iv. Tetraphenylporphyrin (TPP) has a molar absorption coefficient of 4450 M-¹cm-¹ at 532 nm. TPP has a fluorescence quantum yield of 0.11. A 27 µM solution of TPP is irradiated with a 2 µJ pulse of 532 nm laser light in a 1 cm cuvette, how many photons are emitted as fluorescence from the sample.

iv. Tetraphenylporphyrin (TPP) has a molar absorption coefficient of 4450 M-¹cm-¹ at 532 nm. TPP has a fluorescence quantum yield of 0.11. A 27 µM solution of TPP is irradiated with a 2 µJ pulse of 532 nm laser light in a 1 cm cuvette, how many photons are emitted as fluorescence from the sample.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

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iv. Tetraphenylporphyrin (TPP) has a molar absorption coefficient of 4450 M-¹cm-¹
at 532 nm. TPP has a fluorescence quantum yield of 0.11. A 27 µM solution of TPP
is irradiated with a 2 µJ pulse of 532 nm laser light in a 1 cm cuvette, how many
photons are emitted as fluorescence from the sample.

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