One common way to determine phosphorus in urine is to treat the sample, after removing the protein, with Mo (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue colored species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650nm. A 24-hour urine sample was collected and the patient produced 1122mL in 24 hours. A 1.00mL aliquot of the sample was treated with Mo(VI) and ascorbic acid and diluted to a volume of 50.00mL. A calibration curve was prepared by treating 1.00mL aliquots of phosphate standard solutions in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650nm and the following results obtained: Solution Absorbance at 650nm: 1ppm P0.2302ppm P0.4363ppm P0.6384ppm P0.848Urine sample 0.518 How many grams of phosphorus were eliminated per day by the patient?
One common way to determine phosphorus in urine is to treat the sample, after removing the protein, with Mo (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue colored species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650nm. A 24-hour urine sample was collected and the patient produced 1122mL in 24 hours. A 1.00mL aliquot of the sample was treated with Mo(VI) and ascorbic acid and diluted to a volume of 50.00mL. A calibration curve was prepared by treating 1.00mL aliquots of phosphate standard solutions in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650nm and the following results obtained:
Solution Absorbance at 650nm:
1ppm P0.230
2ppm P0.436
3ppm P0.638
4ppm P0.848
Urine sample 0.518
How many grams of phosphorus were eliminated per day by the patient?
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