One common way to determine phosphorous in urine is to treat the sample after removing the protein with molybdenum (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue-coloured species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A 24-hour urine sample was collected, and the patient produced 1122 mL in 24 hours. A 1.00 mL aliquot of the sample was treated with Mo (VI) and ascorbic acid and diluted to a volume of 50.00 mL A calibration curve was prepared by treating 1.00 mL aliquot of phosphate standard solution in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650 nm and the following results were obtained. ppm, P aBSORBANCE (A) 1.003.00 0.230 2.00 0.436 3.00 0.638 4.00 0.848 Urine sample 0.518 ffind the slope and intercept, then graph it.
One common way to determine phosphorous in urine is to treat the sample after removing the protein with molybdenum (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue-coloured species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A 24-hour urine sample was collected, and the patient produced 1122 mL in 24 hours. A 1.00 mL aliquot of the sample was treated with Mo (VI) and ascorbic acid and diluted to a volume of 50.00 mL A calibration curve was prepared by treating 1.00 mL aliquot of phosphate standard solution in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650 nm and the following results were obtained. ppm, P aBSORBANCE (A) 1.003.00 0.230 2.00 0.436 3.00 0.638 4.00 0.848 Urine sample 0.518 ffind the slope and intercept, then graph it.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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One common way to determine phosphorous in urine is to treat the sample after removing the protein with molybdenum (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue-coloured species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A 24-hour urine sample was collected, and the patient produced 1122 mL in 24 hours. A 1.00 mL aliquot of the sample was treated with Mo (VI) and ascorbic acid and diluted to a volume of 50.00 mL A calibration curve was prepared by treating 1.00 mL aliquot of phosphate standard solution in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650 nm and the following results were obtained.
| ppm, P | aBSORBANCE (A) |
| 1.003.00 | 0.230 |
| 2.00 | 0.436 |
| 3.00 | 0.638 |
| 4.00 | 0.848 |
| Urine sample | 0.518 |
ffind the slope and intercept, then graph it.
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