Jsing a fluorescent model substrate, you study the elow data. Write an equation describing reaction v ny constants that you include in your equation.

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4. Using a fluorescent model substrate, you study the kinetics of an enzyme-catalyzed reaction. You observe the
below data. Write an equation describing reaction velocity (v) versus [S]. Define and provide a numerical value for
any constants that you include in your equation.
16
14
12
10
6
4
2
0.0
0.5
1.0
substrate =I
at 50 mM and observe the following
1.5
2.0
2.5
3.0
3.5
[S] / mM
In pilot experiments, you add the putative inhibitor imidazole
H
reaction velocities:
Doesn't affect any thing
[S] = 1 mM 5 µM min-1
[S] = 2 mM 99 µM min-1
[S] = 4 mM 11 µM min-1
Is imidazole a competitive or non-competitive inhibitor? Provide a brief explanation.
v/ µM min-
Transcribed Image Text:4. Using a fluorescent model substrate, you study the kinetics of an enzyme-catalyzed reaction. You observe the below data. Write an equation describing reaction velocity (v) versus [S]. Define and provide a numerical value for any constants that you include in your equation. 16 14 12 10 6 4 2 0.0 0.5 1.0 substrate =I at 50 mM and observe the following 1.5 2.0 2.5 3.0 3.5 [S] / mM In pilot experiments, you add the putative inhibitor imidazole H reaction velocities: Doesn't affect any thing [S] = 1 mM 5 µM min-1 [S] = 2 mM 99 µM min-1 [S] = 4 mM 11 µM min-1 Is imidazole a competitive or non-competitive inhibitor? Provide a brief explanation. v/ µM min-
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