Question: 3 patient samples were run against a 5.0 mmol/L glucose standard using a hexokinase method measured at 340 nm. The standard was run in duplicate. A blank was run. A control was run. The following results were obtained: Identification Standard 1 Standard 2 Control Patient 1 Patient 2 Patient 3 Absorbance 0.312 0.306 0.400 0.301 0.701 1.624 1. Calculate the difference from the mean (estimation of precision) of your 2 standards. 2. If the method requires the standards to be within 10% are the standards acceptable? 3. Calculate the value of your control based on the mean of the absorbances of your standards. 4. If the range for your control is 5.2-6.8 mmol/L is your control acceptable? 5. Calculate the values for the three patient samples. Report the acceptable values. The linearity of the method is 2.0-20.0 mmol/L.

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Question:
3 patient samples were run against a 5.0 mmol/L glucose standard using a hexokinase method measured at 340 nm.
The standard was run in duplicate. A blank was run. A control was run. The following results were obtained:
Identification
Standard 1
Standard 2
Control
Patient 1
Patient 2
Patient 3
Absorbance
0.312
0.306
0.400
0.301
0.701
1.624
1. Calculate the difference from the mean (estimation of precision) of your 2 standards.
2. If the method requires the standards to be within 10% are the standards acceptable?
3. Calculate the value of your control based on the mean of the absorbances of your standards.
4. If the range for your control is 5.2-6.8 mmol/L is your control acceptable?
5. Calculate the values for the three patient samples. Report the acceptable values. The linearity of the method
is 2.0-20.0 mmol/L.
Transcribed Image Text:Question: 3 patient samples were run against a 5.0 mmol/L glucose standard using a hexokinase method measured at 340 nm. The standard was run in duplicate. A blank was run. A control was run. The following results were obtained: Identification Standard 1 Standard 2 Control Patient 1 Patient 2 Patient 3 Absorbance 0.312 0.306 0.400 0.301 0.701 1.624 1. Calculate the difference from the mean (estimation of precision) of your 2 standards. 2. If the method requires the standards to be within 10% are the standards acceptable? 3. Calculate the value of your control based on the mean of the absorbances of your standards. 4. If the range for your control is 5.2-6.8 mmol/L is your control acceptable? 5. Calculate the values for the three patient samples. Report the acceptable values. The linearity of the method is 2.0-20.0 mmol/L.
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