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Is your presumptive Staphylococcus a potential pathogen? • Deoxyribonuclease (DNase) agar • What exoenzyme does this plate test for? • What is an exoenzyme? Uninoculated • Name the indicator. • Name the substrate present in the agar that will be broken down by the enzyme. Give describe what a positive and negative reaction look like. 24-48 hour incubation Methyl green DNASE agar used in the lab does not need HCI • Is your sample DNAse + or DNASE -?

Is your presumptive Staphylococcus a potential pathogen? • Deoxyribonuclease (DNase) agar • What exoenzyme does this plate test for? • What is an exoenzyme? Uninoculated • Name the indicator. • Name the substrate present in the agar that will be broken down by the enzyme. Give describe what a positive and negative reaction look like. 24-48 hour incubation Methyl green DNASE agar used in the lab does not need HCI • Is your sample DNAse + or DNASE -?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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which type of hemolysis your staphylococcus is alpha beta or gamma?

Is your sample DNAse + or DNAse -?

**Is your presumptive Staphylococcus a potential pathogen?** - **Deoxyribonuclease (DNase) agar** - **What exoenzyme does this plate test for?** - What is an exoenzyme? - **Name the indicator.** - **Name the substrate present in the agar that will be broken down by the enzyme.** - **Describe what a positive and negative reaction look like.** - **Is your sample DNase + or DNase -?** **Images and Descriptions:** - **Uninoculated Plate:** Shows a DNase agar plate that is not streaked with bacteria. The plate appears uniformly green. - **24-48 hour incubation:** An image of a DNase agar plate after 24-48 hours of incubation with bacteria. The plate features clear zones around the bacterial streaks. **Explanation:** - **Methyl Green DNase agar:** This is the type of agar used in the lab for testing. It does not require the addition of HCl for interpretation of results. In a DNase test, a positive reaction typically shows a clear zone around the bacterial growth, indicating DNase activity, while a negative reaction generally shows no clearing.
Transcribed Image Text:**Is your presumptive Staphylococcus a potential pathogen?** - **Deoxyribonuclease (DNase) agar** - **What exoenzyme does this plate test for?** - What is an exoenzyme? - **Name the indicator.** - **Name the substrate present in the agar that will be broken down by the enzyme.** - **Describe what a positive and negative reaction look like.** - **Is your sample DNase + or DNase -?** **Images and Descriptions:** - **Uninoculated Plate:** Shows a DNase agar plate that is not streaked with bacteria. The plate appears uniformly green. - **24-48 hour incubation:** An image of a DNase agar plate after 24-48 hours of incubation with bacteria. The plate features clear zones around the bacterial streaks. **Explanation:** - **Methyl Green DNase agar:** This is the type of agar used in the lab for testing. It does not require the addition of HCl for interpretation of results. In a DNase test, a positive reaction typically shows a clear zone around the bacterial growth, indicating DNase activity, while a negative reaction generally shows no clearing.
**Results of CNA Plate after Incubation:** - **Determine Hemolysis** - How do you know which type of hemolysis your Staphylococcus is? - **Determine Novobiocin Sensitivity** - How do you measure a zone of inhibition? - What size is the zone of inhibition? - Is the presumptive Staph sensitive or resistant to novobiocin? - How many mm is considered sensitive? **Diagram Explanation:** The image on the right shows a CNA (Colistin Nalidixic Acid) agar plate after incubation. There is a clear zone labeled "22 mm" around a disc representing the zone of inhibition. This indicates the area where bacterial growth has been inhibited, suggesting sensitivity to the antibiotic novobiocin.
Transcribed Image Text:**Results of CNA Plate after Incubation:** - **Determine Hemolysis** - How do you know which type of hemolysis your Staphylococcus is? - **Determine Novobiocin Sensitivity** - How do you measure a zone of inhibition? - What size is the zone of inhibition? - Is the presumptive Staph sensitive or resistant to novobiocin? - How many mm is considered sensitive? **Diagram Explanation:** The image on the right shows a CNA (Colistin Nalidixic Acid) agar plate after incubation. There is a clear zone labeled "22 mm" around a disc representing the zone of inhibition. This indicates the area where bacterial growth has been inhibited, suggesting sensitivity to the antibiotic novobiocin.
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