initiation sequences Operator (O)- sites termination sequence Ribosome- Bacterial binding site promoter (P) sequences Gene-encoding repressor that binds O and regulates P ori Selectable genetic marker (e.g., antibiotic resistance)
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Explain the function of selectable genetic markers and why they should be contained.
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- 24An ade+ arg+ cys+ his+ leu+ pro+ bacterial strain is knownto be lysogenic for a newly discovered phage, but the siteof the prophage is not known. The bacterial map isleucysarghisadeproThe lysogenic strain is used as a source of the phage, andthe phages are added to a bacterial strain of genotypeade- arg- cys- his- leu- pro-. After a short incubation,samples of these bacteria are plated on six differentmedia, with the supplementations indicated in thefollowing table. The table also shows whether colonieswere observed on the various media.PresenceMedium Ade Arg Cys His Leu Pro of colonies1 - + + + + + N2 + - + + + + N3 + + - + + + C4 + + + - + + N5 + + + + - + C6 + + + + + - NNutrient supplementation in medium(In this table, a plus sign indicates the presence of anutrient supplement, a minus sign indicates that asupplement is not present, N indicates no colonies, and Cindicates colonies present.)a. What genetic process is at work here?b. What is the approximate locus of the prophage?Generation of induced pluripotent stem (iPS)cells was first accomplished using retroviral vectors tocarry the OSKM (Oct4, Sox2, Klf4, and Myc) set of tran-scription regulators into cells. The efficiency of fibroblastreprogramming was typically low (0.01%), in part becauselarge numbers of retroviruses must integrate to bringabout reprogramming and each integration event carrieswith it the risk of inappropriately disrupting or activatinga critical gene. In what other ways, or other forms, do yousuppose you might deliver the OSKM transcription regula-tors so as to avoid these problems?
- Before development of a vaccine against this microbe, thedisease it caused accounted for two-thirds of bacterial meningi-tis cases during the first year of life but is still the number oneleading cause of mental retardation in patients who survive seri-ous disease due to permanent central nervous system disorders.What is the microorganism?(a) Haemophilus influenzae type B(b) Haemophilus influenzae type A(c) Neisseria meningitidis(d) Streptococcus pneumoniae(e) Listeria monocytogenesPlz asap with conclusionPlease help Olivia is an ER soluble resident protein that has ended up at the Cis-Golgi network. Explain: - Why this protein leaves the ER - Why it must be retrieved - How it is retrieved
- Name conditions caused by abnormalities of vertical linkages and horizontal (lateral) linkages in RBC transmembrane and cytoskeletal proteins.When challenged with a low oxygen environment, knownas hypoxia, the body produces a hormone called erythropoietin(EPO), which then stimulates red blood cell production tocarry more oxygen. Transcription of the gene encoding EPO isdependent upon the hypoxia-inducible factor (HIF), which is atranscriptional activator. However, HIF alone is not sufficientto activate EPO. For example, Wang et al. (2010. PLOS ONE 5:e10002) showed that HIF recruits another protein called p300to an enhancer for the EPO gene. Furthermore, deletion of p300significantly impaired transcription of the EPO gene in responseto hypoxia. Given that p300 is a type of histone acetyl transferase,how might p300 influence transcription of the EPO gene?Some explanation for this image.
- What do you mean by Use of Dexamethasone in the context of COVID 19? Please add diagrams or figures supporting your answer. Please briefly explain at your own easy words. I will rate you positive if you do so. Please don't use AI for answering this question.you develop the details on the stepwise design. Can a siRNA-based technology to treat COVID-19? If so, please provideGive typed full explanation