Q: What is the polypeptide that will be formed from the following DNA sequence? Template DNA…
A: In molecular biology complementary base pairing or complementarity is a reletion between the two…
Q: In figure 10.16 , how would the curve appear if the GC content of the DNA sample were increased? How…
A: Introduction: DNA is made of four nucleotide bases that are adenine (A), thymine (T), guanine (G),…
Q: In ONE sentence define the function of the following 1. SSBS = The SSBS are single 2. Beta subunit…
A: These are all the enzymes used in replication of DNA Replication of DNA is a very important process…
Q: In DNA extraction what else might be in the ethanol/aqueous interface? How could you eliminate it
A: Monovalent cations and ethanol play a key role in removing the solvation shell that surrounds DNA,…
Q: In the "Indexing DNA" method of n k-mer table, the maximum number of compares when the k-mers are…
A: Indexing of genome is done in order to index all the small sequenes within the large genome.The…
Q: A facility says they need 15 μL of a 40 ng/μL solution of plasmid DNA for sequencing. The typical…
A: Appropriate amount of DNA is one the key elements in gene sequencing. Amount of DNA for sequencing…
Q: In Noll’s experiment to test the beads-on-a-string model, exposure of nuclei to a low concentration…
A: Markus Noll's experiment to test the Kornberg's model of Nucleosome, also known as beads-on-string…
Q: How would describe the observation of DNA precipitated out of the water-ethanol solution
A: Ethanol precipitation is a process where ethanol is used as an anti-solvent to purify and…
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: DNA (Deoxyribonucleic acid) and RNA are two examples of nucleic acids (Ribonucleic acid).…
Q: During a typical gel electrophoresis set up (negative pole at the top), which DNA fragment would be…
A: Gel electrophoresis is the technique for the separation of DNA fragments on the basis of size and…
Q: Why are the first 20 bases typically ignored from a Sanger sequencing .ab1 file? The data quality is…
A: The data quality is too low and the data is noisy
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: Given values: Total DNA extracted = 500 μL Total weight of wheat used for DNA extraction = 200 mg…
Q: Which of the following base-pairing rule is correct : * Adenine with guanine and thymine with…
A: DNA is a deoxyribose nucleic acid. it contains deoxyribose sugar, nitrogenous base Purine, and…
Q: onsidering the number of base pairs, compute for the actual length (in micrometers) of this DN
A: There are 7 base pairs. Length of one base pair is 3.4 Angstrom. Length of 7 base pairs is 3.4 X 7=…
Q: Why is it important that the alcohol used in the DNA extraction is kept cold?
A: The ice cold alcohol, helps to isolate the DNA from a solution.
Q: Which of the folllowing is the term used for ultra compact form of DNA coiling? a. supercoiling…
A: Compacting DNA is important for packaging it within the cells. As the length of a DNA molecule can…
Q: What is the purpose of adding table salt (NaCl) to the DNA extraction buffer? Table salt dissociates…
A: DNA is a double-helical structure whose backbone is made up of sugar-phosphate and bases are present…
Q: Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA…
A: DNA extraction procedure refers to the methodology that is followed to purify the the DNA (cellular…
Q: oner micro mole of 48 nulceotide dna was synthesised via solid phase .this sample contains salts…
A: DNA (deoxyribonucleic acid) is the polymer of nucleotides joined by a phosphodiester linkage. Each…
Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…
A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…
Q: DNA sample which is approximately 600 base pairs long. What percentage of Agarose would you make…
A: The biochemical molecule that is built up with two polynucleotide chains is called DNA…
Q: The electrical current created during gel electrophoresis forces DNA to move from the negative end…
A: Answer. DNA or Deoxyribonucleic acid is made up of nucleotides. A nucleotide contains a phosphate…
Q: Identify the CORRECT sequence of processes that takes place during DNA extraction. - ethanol…
A: Introduction : DNA: DNA Extraction Is A Method For Isolating DNA From A Biological Sample.…
Q: What is the use of Liquid soap in DNA extraction?* A. binds cell membrane and nuclei B. dissolves…
A: DNA extraction is a process of extracting DNA from the cell for further experiments. It is a method…
Q: Which of the following best describes the process of DNA seqencing.
A: DNA is mainly made up of the nucleotide and also contains genetic information in the genes. Every…
Q: Please draw the DNA denaturation (DNA melting) curves corresponding to the three molecules and…
A: DNA denaturation is the breaking down of DNA molecule, for comparison or sequencing. DNA can be…
Q: The ratio of (A+T) to (G+C) in a particular DNA is 1.0. Is this sufficient data to determine…
A: Deoxyribonucleic acid (DNA) is the molecule. that contains the complete genetic code of an organism
Q: Sanger DNA sequencing/ dideoxy sequencing was used as shown in the diagram below. The arrow…
A: Sanger's sequencing method. It is a method based on DNA synthesis. In normal DNA synthesis,…
Q: non-coding DNAs can be put into two groups
A: Part of DNA that does not encode a protein sequence is called as non coding DNA. tRNA, rRNA and…
Q: Which of the mechanisms efficiently and effectively organize the DNA into a usable form?…
A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around one…
Q: Similarities between DNA agarose gels and SDS-PAGE are:
A: Gel electrophoresis is the technique of separation of molecules of the basis of their size and…
Q: Open the bag of fruit and add 2 teaspoons of your DNA extraction buffer. What are cell membranes…
A: Cell is a structural and functional unit of all organisms. The cell was discovered by Robert Hooke…
Q: What is the purpose of adding table salt (NaCl) to the DNA extraction buffer? 1.Table salt…
A: The DNA extraction of procedure for isolating DNA from the cells and it is the routine procedure…
Q: If you have five test tubes each containing a double stand DNA, which of the following double…
A: Answer- The denaturation of DNA is depend on the melting temperature and the composition of the…
Q: DNA in an open configuration is common in the darkly staining regions called heterochromatin. True…
A: Deoxyribonucleic acid (DNA) is a polymer made up of two polynucleotide chains which wrap around…
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: Given wheat 200 milligram germ extraction volume. = 500 microlitre Sample used for analysis=…
Q: You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500…
A: DNA generally stands for Deoxyribonucleic acid It is generally double standard Purine and…
Q: The sum of the lengths of DNA fragments 1-3 equal the total original length? True / False
A: Answer : The sum of the lengths of DNA fragments 1-3 equal the total original length : false.
Q: Which of the following caps contains two methylated bases? a. 0 b. 1 c. 2 d. 3
A: mRNA cap is defined as the modification at the 5' end of the RNA polymerase II-transcribed RNA. This…
Q: Which is the odd one out ? explain how the rest are connected. primers dNTPs DNA polymerase…
A: The polymerase chain reaction (PCR) is a widely used method for rapidly making millions to billions…
Q: TRUE OR FALSE a) The 2 chains composing one double helix run in opposite directions; they are…
A: All living organisms are made up of cells, which are the most basic unit of life. They are…
Q: In the "Indexing DNA" method of n k-mer table, the maximum number of compares when the k-mers are…
A: The process of indexing a genome is similar to that of indexing a book.
Q: How many phosphates, deoxyribose and nitrogenous bases are there in a DNA that has 15
A: DNA is a polymer of nucleotides. Two DNA strands lie side by side with their complementary bases…
Q: During a typical gel electrophoresis set up (negative pole at the top), which DNA fragment would be…
A: The answer is 100 kb .
Q: In complementary base pairing which of the following base parings is incorrect? a. G-C b. G-T OC.…
A: ANSWER'- b) G-T Explain;-G-T is incorrect pair. Base pairing happens between a purine and…
Q: Which of the following substances adds equivalent DNA nucleotides to the 3' end of Okazaki fragments…
A: DNA polymerase I
Q: Using Chargaff’s rule of base pairing determine the amount of guanine in 120 bp long fragment of…
A: According to Chargaff's rules, DNA from every species of any organism should have a 1:1…
Q: DNA of different densities is distinguished by a procedure called caesium chloride (CsCI) density…
A: Cesium chloride (CsCI) density centrifugation generates a gradient of cesium and chloride ions.…
In which of the following is DNA soluble or more soluble
- Water
- Ethanol or ethyl alcohol
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- The structure given is uridylate deoxycytidylate thymidylic acid uridine monophosphateOur laboratory has a stock of 50 X TAE (Tris-Acetate) and the laboratory manager asks you to prepare a 2% gel using 1XTAE that you will prepare from agarose and stock. How do you prepare? (tank tray volume 250 ml).The structure given is NH OH 'N' HO-P=0 ÓH A uridine monophosphate deoxycytidylate C thymidylic acid uridylate
- An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.You want to prepare a 15 mL gel containing 0.8% (weight /volume) agarose. How much agarosedo you need to weigh to prepare the solution? Show all your calculations.Hint: Write down the units for 0.8% knowing that agarose is a powder that is mixed with aliquid
- Classify the following nitrogenous bases into purines or pyrimidines. NH2 NH2 CH CH HN -NH HN CH -NH EN CH NH2 H Guanine Cytosine Uracil Adenine Thymine Purine PyrimidineThese are found in soybean oil (select all that apply). Polyunsaturated fatty acids Linoleic acid Oleic acid Monounsaturated fatty acid Alpha-linolenic acid FlavonoidsIf you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.