Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260o) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below

You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A260o) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question
What to Calculate Calculation / Result DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40%
Transcribed Image Text:What to Calculate Calculation / Result DNA concentration in cuvette solution (in microgram per ml) Dilution factor of DNA sample in cuvette Total amount (microgram) of DNA in the entire extraction sample Total amount (milligram) of DNA in 100g wheat germ assuming an extraction efficiency of 40%
You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A26o) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below
Transcribed Image Text:You extract DNA from 200 milligram of wheat germ. Your total volume of DNA extraction sample is 500 microlitres. You pipette 200 microlitres of this extraction sample into a 3-ml cuvette and make up to 3.0 ml using buffer. The absorbance at 260 nm (A26o) of the solution in the cuvette is 0.2 (Remember: an A260 of 1.0 corresponds to a DNA concentration in the cuvette solution of 50 micrograms per ml). Calculate the total amount of DNA in 100g wheat germ, assuming that the extraction efficiency of DNA from wheat germ during your lab protocol is 40%. Please fill out each of the boxes in the Table below
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 4 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS