Identify the CORRECT sequence of processes that takes place during DNA extraction. - ethanol washing. - ethanol washing. ethanol washing. ethanol precipitation A. Cell lysis - salt precipitation B. Cell lysis SDS treatment salt precipitation - ethanol precipitation cell lysis SDS treatment salt precipitation C. Ethanol precipitation -SDS treatment - D. Ethanol precipitation - SDS treatment - salt precipitation - ethanol washing - cell lysis.

Q: Please choose the correct answer. Initial DNA nucleotides are added by reverse transcriptase from…

A: Reverse transcriptase is RNA dependent DNA polymerase. It transcribes ssRNA into DNA.

Q: Electrophoretic-Mobility Shift Assay DNA affinity chromatography Immunoprecipitation DNA footprint…

A: Introduction  DNA replication is the process by which a cell makes a copy of its genetic material,…

Q: 1.DNA helicase 2.SSBP 3.DNA ligase 4.Primase 5.DNA Polymerase III A. Synthesizes RNA primer B.…

A: Deoxyribonucleic Acid (DNA), is a complex organic molecule is the hereditary material that carries…

Q: Define the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd.…

A: Recombinant DNA is made by combining two or more DNA from other sources. The process depends on the…

Q: DNA polymerase δ: DNA polymerase III as ________ a. Helicase: RPA b. DNA ligase: DNA polymerase I…

A: a) RPA stimulates helicase-catalyzed DNA polymerase III unwinding, enlists translocases to sites of…

Q: Which of the following sequence shows the correct order of the steps involved in Recombinant DNA…

A: Recombinant DNA (rDNA) technology is a set of techniques that allows the combination of DNA…

Q: A. Avery, MacLeod and McCarty proved DNA is the genetic material. If you were to repeat their…

A: The DNA replication is heterocatalytic process by which a new DNA strand is synthesized on a old DNA…

Q: Write down the causes and solutions of the following problems encountered in DNA isolation. 1- low…

A: LOW DNA CONCENTRATION. The common cause for low DNA concentration are poor culturing…

Q: Link the technique to the correct enzyme.…

A:

Q: a.       The importance of recombinant DNA technology in Environment  b.       Potential products…

A: DNA : Deoxyribo nucleic acid. It is a polymer which is composed of two polynucleotide chains that…

Q: The DNA fragments generated during a cycle sequencing reaction are separated by sizes using…

A: The detection of DNA sequence is a tough process and requires lot of patience.  DNA sequence…

Q: What should be the first step in the protocol for the purification of DNA from any source (including…

A: DNA is a nucleic acid found in the nucleus of the eukaryotic cells. It acts as genetic material. DNA…

Q: The purpose of using a washing soap in DNA extraction experiment is to

A: Washing soap is made of salts of fatty acids which dissolves the plasma membrane by solubilization…

Q: which sample shows the plasmid is fully digested

A: 50 bp ladder sequence : 500 bp 450 bp 400 bp 350 bp 300 bp 250 bp 200 bp 150 bp 100 bp 50 bp

Q: Choose

A: Introduction :- DNA fingerprinting is a method used to identify an individual from a sample of DNA…

Q: To relax a supercoiled DNA, you may use, Select one: O a. DNA topoisomerase I O b. DNA helicase O c.…

A: Ans:- (a) DNA topoisomerase I

Q: Gel electrophoresis can be used to separate DNA on the basis of:   A. the probe used.   B. size.…

A: Electrophoresis is a laboratory technique used to separate DNA, RNA or protein molecules. Agarose…

Q: What are the roles of the following reagents in DNA extraction? a. Ethanol b. NaCl c. SDS d. TE…

A: A) The initial role of the ethanol and monovalent cations is to remove the solvation shell…

Q: Which DNA fragment is e smallest? Negative End #1 12 13 <14 Positive End

A: Answer: AGAROSE GEL ELECTROPHORESIS = This is a technique which we use to separate different…

Q: The RNA primer at the beginning of each Okazaki fragment is removed by

A: Introduction: The small pieces of the discontinuously synthesized DNA are basically known as Okazaki…

Q: After performing a PCR reaction with an expected product size of 700 bp, you observe the results…

A: PCR stands for a polymerase chain reaction and it is a technique used to amplify the DNA sample…

Q: Agriculture  a.       The importance of recombinant DNA technology in Agriculture  b.…

A: Recombinant DNA technology is a combination of many laboratory techniques which results in…

Q: In order to determine the purity of a DNA sample, spectrophotometry can be carried our at Answer to…

A: Spectrophotometry is the quantitative analysis of spectra to compare the relative absorption or…

Q: Question 9. RT-PCR represents an adaptation of PCR to the investigation of RNA. What enzyme is used…

A: A given DNA segment may be quickly multiplied (amplified) into a multitude of copies using the…

Q: 2. why contaminants must be avoided in DNA samples?

A: DNA contamination occurs when foreign DNA contaminates your intended DNA sample. DNA contamination…

Q: Which of the following is false about the types of DNA? A. Z-DNA is right handed double helix. B.…

A: Nucleic acids are the organic materials present in all organisms in the form of DNA or RNA. Nucleic…

Q: Choose the combination of answers that most accurately completes the statement.Which of the…

A: Three major steps are involved in polymerase chain reaction are denaturing, annealing and extension.…

Q: The typical cycle of a PCR reaction includes a period of time at 95 degrees, 55 degrees, and 72…

A: PCR which is also called as Polymerase Chain Reaction is a method used in DNA amplification of…

Q: The figure below shows an absorption spectrum of DNA obtained using NanoDrop. Is this DNA…

A: Option B Contaminated with RNA

Q: In one PCR cycle, the correct order of steps isa. primer annealing, primer extension,…

A: PCR refers to the polymerase chain reaction. It amplifies the small amount of DNA into several…

Q: . The concept of (CODIS, multiplexing) involves simultaneous detection of more than one DNA marker.

A: This is called Multiplexing.

Q: 1. what are the three main steps of a solid phase DNA extraction? Answer is Lysis, but does not…

A: “Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Pyrimidine dimers in DNA may be repaired by in E. coli cells. (select all correct answers) Select…

A: The most well-known DNA lesion involving a single DNA strand is pyrimidine dimer. It is an…

Q: After using the blender to blend the split peas, water, and salt mixture, cheesecloth is used to. .…

A: It is a cotton cloth that is loosely woven and resembles gauze.

Q: All of the following contribute to a low mutation rate EXCEPT a. high fidelity of DNA polymerase…

A: A mutation is a change in the DNA sequence of a gene, chromosome, or entire genome. This can result…

Q: 8. B. 25mL of 1% Sodium Bicarbonate solution was created and the ph was taken. Whi value below…

A: Note: Please upload your other question separately as per answering guidiline, tutors can answer a…