B. Genome Mining 1. Using the sequences from the previous activity, identify the organisms to which those DNA sequences belong to. 2. To determine the organisms to which these sequences belong, perform the nucleotide BLAST or blastn search. 3. 4. You are going to use the internet to proceed. Visit the NCBI website or just click on the link below. https://blast.ncbi.nlm.nih.gov/Blast.cgi 5. In the Enter Query Sequence box, please input the DNA sequences of the unknown organism which you have determined. 6. Then, press BLAST button. 7. Once the result is out, find out the name of the species which on top of the lists of sequences producing significant alignment. 8. Write down the name of the species. 9. Do BLAST search again with the rest of the Unknown. A. DNA Sequencing 1. Determine the base sequences of the sample DNA from unknown organisms by examining the bands after gel electrophoresis on photographic films. a. Unknown 1 b. Unknown 2 AGT C I I c. Unknown 3 ||| || | | AGT | || | ||| | | | || I || || ull с I AGT 2. Write down the DNA sequences of each Unknown. I I || d. Unknown 4 || |||||| AGT || ||| | || I || ||| с C ||
B. Genome Mining 1. Using the sequences from the previous activity, identify the organisms to which those DNA sequences belong to. 2. To determine the organisms to which these sequences belong, perform the nucleotide BLAST or blastn search. 3. 4. You are going to use the internet to proceed. Visit the NCBI website or just click on the link below. https://blast.ncbi.nlm.nih.gov/Blast.cgi 5. In the Enter Query Sequence box, please input the DNA sequences of the unknown organism which you have determined. 6. Then, press BLAST button. 7. Once the result is out, find out the name of the species which on top of the lists of sequences producing significant alignment. 8. Write down the name of the species. 9. Do BLAST search again with the rest of the Unknown. A. DNA Sequencing 1. Determine the base sequences of the sample DNA from unknown organisms by examining the bands after gel electrophoresis on photographic films. a. Unknown 1 b. Unknown 2 AGT C I I c. Unknown 3 ||| || | | AGT | || | ||| | | | || I || || ull с I AGT 2. Write down the DNA sequences of each Unknown. I I || d. Unknown 4 || |||||| AGT || ||| | || I || ||| с C ||
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:B. Genome Mining
1.
Using the sequences from the previous activity, identify the organisms to which those
DNA sequences belong to.
2.
To determine the organisms to which these sequences belong, perform the nucleotide
BLAST or blastn search.
3.
4.
You are going to use the internet to proceed.
Visit the NCBI website or just click on the link below.
https://blast.ncbi.nlm.nih.gov/Blast.cgi
5.
In the Enter Query Sequence box, please input the DNA sequences of the unknown
organism which you have determined.
6.
Then, press BLAST button.
7. Once the result is out, find out the name of the species which on top of the lists of
sequences producing significant alignment.
8. Write down the name of the species.
9. Do BLAST search again with the rest of the Unknown.
Transcribed Image Text:A. DNA Sequencing
1. Determine the base sequences of the sample DNA from unknown organisms by
examining the bands after gel electrophoresis on photographic films.
a. Unknown 1
b. Unknown 2
AGT C
I
I
c. Unknown 3
|||
|| | |
AGT
| ||
| |||
| | | ||
I
||
||
ull
с
I
AGT
2. Write down the DNA sequences of each Unknown.
I
I
||
d. Unknown 4
||
||||||
AGT
|| |||
| ||
I
||
|||
с
C
||
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